Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 618270..619006 Replicon chromosome
Accession NZ_CP061062
Organism Klebsiella pneumoniae strain HKP0018

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag IBX29_RS02990 Protein ID WP_032433360.1
Coordinates 618524..619006 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag IBX29_RS02985 Protein ID WP_003026799.1
Coordinates 618270..618536 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IBX29_RS02960 613916..615055 + 1140 WP_032433368.1 mannitol dehydrogenase -
IBX29_RS02965 615084..615746 + 663 WP_032433366.1 dihydroxyacetone kinase subunit L -
IBX29_RS02970 615730..616734 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
IBX29_RS02975 616752..617384 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
IBX29_RS02980 617394..617957 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
IBX29_RS02985 618270..618536 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
IBX29_RS02990 618524..619006 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
IBX29_RS02995 619164..620696 - 1533 WP_015065592.1 IS3-like element ISKpn38 family transposase -
IBX29_RS03000 620808..622211 + 1404 WP_039815096.1 ISNCY-like element ISKpn21 family transposase -
IBX29_RS03005 622240..622872 - 633 WP_001567369.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 589082..635151 46069


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T171991 WP_032433360.1 NZ_CP061062:618524-619006 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T171991 NZ_CP079139:c245571-245089 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGGAATGAGAGCGCCTGAATCCCTGACGGCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT171991 WP_003026799.1 NZ_CP061062:618270-618536 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT171991 NZ_CP079139:c245840-245562 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References