Detailed information of TA system TA171990

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 601579..602222
• Replicon: chromosome
• Accession: NZ_CP061062
• Organism: Klebsiella pneumoniae strain HKP0018

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A4S8C081
• Locus tag: IBX29_RS02895
• Protein ID: WP_032433387.1
• Coordinates: 601806..602222 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: IBX29_RS02890
• Protein ID: WP_001261276.1
• Coordinates: 601579..601809 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IBX29_RS02875	596601..597688	+	1088	Protein_556	transcriptional regulator	-
IBX29_RS02880	597691..599931	+	2241	WP_045326788.1	NTPase	-
IBX29_RS02885	600459..601274	-	816	WP_032433388.1	hypothetical protein	-
IBX29_RS02890	601579..601809	+	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IBX29_RS02895	601806..602222	+	417	WP_032433387.1	type II toxin-antitoxin system VapC family toxin	Toxin
IBX29_RS02900	602378..603358	+	981	WP_032433385.1	hypothetical protein	-
IBX29_RS02905	603553..605124	-	1572	WP_032433383.1	ATP-binding protein	-
IBX29_RS02910	605443..605691	+	249	WP_032433382.1	hypothetical protein	-
IBX29_RS02915	605750..606268	+	519	WP_045326794.1	hypothetical protein	-
IBX29_RS02920	606299..606790	+	492	WP_032433378.1	hypothetical protein	-
IBX29_RS02925	606850..607053	+	204	WP_032433376.1	hemolysin expression modulator Hha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	589082..635151	46069

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14974.43 Da
• Isoelectric Point: 7.8921

>T171990 WP_032433387.1 NZ_CP061062:601806-602222 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALHLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLTLEDWVI

Nucleotide

• Length: 417 bp

>T171990 NZ_CP079139:168129-168599 [Klebsiella pneumoniae subsp. pneumoniae]
GTGATCTTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT171990 WP_001261276.1 NZ_CP061062:601579-601809 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT171990 NZ_CP079139:167689-168132 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A4S8C081

Antitoxin

• AlphaFold DB: A0A387K023