Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5377077..5377702 Replicon chromosome
Accession NZ_CP061053
Organism Klebsiella pneumoniae strain HKP0067

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag IBX31_RS26005 Protein ID WP_002882817.1
Coordinates 5377077..5377460 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IBX31_RS26010 Protein ID WP_004150355.1
Coordinates 5377460..5377702 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IBX31_RS25990 5374443..5375345 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
IBX31_RS25995 5375342..5375977 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
IBX31_RS26000 5375974..5376903 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IBX31_RS26005 5377077..5377460 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
IBX31_RS26010 5377460..5377702 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
IBX31_RS26015 5377907..5378824 + 918 WP_021466676.1 alpha/beta hydrolase -
IBX31_RS26020 5378838..5379779 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
IBX31_RS26025 5379824..5380261 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
IBX31_RS26030 5380258..5381118 - 861 WP_004146232.1 virulence factor BrkB family protein -
IBX31_RS26035 5381112..5381711 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T171989 WP_002882817.1 NZ_CP061053:c5377460-5377077 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T171989 NZ_CP079139:23293-23577 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACCATTTAACCAAACACAGAAATTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT171989 WP_004150355.1 NZ_CP061053:c5377702-5377460 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT171989 NZ_CP079139:23055-23303 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCGGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGTTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References