Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1472301..1473037 Replicon chromosome
Accession NZ_CP061053
Organism Klebsiella pneumoniae strain HKP0067

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag IBX31_RS07270 Protein ID WP_032433360.1
Coordinates 1472555..1473037 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag IBX31_RS07265 Protein ID WP_003026799.1
Coordinates 1472301..1472567 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IBX31_RS07240 1467947..1469086 + 1140 WP_032433368.1 mannitol dehydrogenase -
IBX31_RS07245 1469115..1469777 + 663 WP_032433366.1 dihydroxyacetone kinase subunit L -
IBX31_RS07250 1469761..1470765 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
IBX31_RS07255 1470783..1471415 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
IBX31_RS07260 1471425..1471988 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
IBX31_RS07265 1472301..1472567 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
IBX31_RS07270 1472555..1473037 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
IBX31_RS07275 1473194..1474725 - 1532 Protein_1421 IS3 family transposase -
IBX31_RS07280 1474837..1476239 + 1403 Protein_1422 ISNCY family transposase -
IBX31_RS07285 1476268..1476902 - 635 Protein_1423 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1441164..1489193 48029


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T171975 WP_032433360.1 NZ_CP061053:1472555-1473037 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T171975 NZ_CP079131:c4665509-4665192 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT171975 WP_003026799.1 NZ_CP061053:1472301-1472567 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT171975 NZ_CP079131:c4665199-4664912 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References