Detailed information of TA system TA171952

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3660491..3661712
• Replicon: chromosome
• Accession: NZ_CP061034
• Organism: Pseudomonas aeruginosa strain PA3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EBO27_RS17020
• Protein ID: WP_003114949.1
• Coordinates: 3661134..3661712 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: EBO27_RS17015
• Protein ID: WP_003088112.1
• Coordinates: 3660491..3661045 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EBO27_RS16990	3656059..3656610	+	552	WP_003102727.1	XRE family transcriptional regulator	-
EBO27_RS16995	3656607..3657005	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
EBO27_RS17000	3657018..3657341	-	324	WP_003162861.1	multidrug efflux SMR transporter	-
EBO27_RS17005	3657672..3658133	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
EBO27_RS17010	3658138..3660333	+	2196	WP_004349295.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
EBO27_RS17015	3660491..3661045	+	555	WP_003088112.1	helix-turn-helix domain-containing protein	Antitoxin
EBO27_RS17020	3661134..3661712	+	579	WP_003114949.1	HD domain-containing protein	Toxin
EBO27_RS17025	3661820..3663007	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
EBO27_RS17030	3662997..3665168	-	2172	WP_009876279.1	type I secretion system permease/ATPase	-
EBO27_RS17035	3665158..3666435	-	1278	WP_003122808.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21897.75 Da
• Isoelectric Point: 5.3655

>T171952 WP_003114949.1 NZ_CP061034:3661134-3661712 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAVRRDA

Nucleotide

• Length: 579 bp

>T171952 NZ_CP079116:c161654-161349 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT171952 WP_003088112.1 NZ_CP061034:3660491-3661045 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT171952 NZ_CP079116:c161874-161656 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA