Detailed information of TA system TA171951

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 2450790..2451584
• Replicon: chromosome
• Accession: NZ_CP061034
• Organism: Pseudomonas aeruginosa strain PA3

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A844NUK8
• Locus tag: EBO27_RS11680
• Protein ID: WP_021263385.1
• Coordinates: 2451405..2451584 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: EBO27_RS11675
• Protein ID: WP_021263386.1
• Coordinates: 2450790..2451365 (-)
• Length: 192 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EBO27_RS11640	2446034..2446606	-	573	WP_023088260.1	S24 family peptidase	-
EBO27_RS11645	2446983..2447201	+	219	WP_003451709.1	helix-turn-helix domain-containing protein	-
EBO27_RS11650	2447233..2447805	+	573	WP_033987544.1	hypothetical protein	-
EBO27_RS11655	2447808..2448818	+	1011	WP_054375713.1	hypothetical protein	-
EBO27_RS11660	2448946..2449359	+	414	WP_034061691.1	hypothetical protein	-
EBO27_RS11665	2449352..2449801	+	450	Protein_2304	RusA family crossover junction endodeoxyribonuclease	-
EBO27_RS11670	2449830..2450699	+	870	WP_150035569.1	hypothetical protein	-
EBO27_RS11675	2450790..2451365	-	576	WP_021263386.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EBO27_RS11680	2451405..2451584	-	180	WP_021263385.1	type II toxin-antitoxin system HicA family toxin	Toxin
EBO27_RS11685	2451673..2451888	+	216	WP_054375712.1	hypothetical protein	-
EBO27_RS11690	2452031..2452420	+	390	WP_031673411.1	hypothetical protein	-
EBO27_RS11695	2452413..2452697	+	285	WP_054375711.1	phage holin family protein	-
EBO27_RS11700	2452706..2453305	+	600	WP_031673408.1	terminase small subunit	-
EBO27_RS11705	2453289..2454584	+	1296	WP_054375710.1	terminase family protein	-
EBO27_RS11710	2454587..2455942	+	1356	WP_054375709.1	DUF4055 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2419811..2473968	54157

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6591.62 Da
• Isoelectric Point: 11.1475

>T171951 WP_021263385.1 NZ_CP061034:c2451584-2451405 [Pseudomonas aeruginosa]
MKFSEFRRWLRAQGVSFEAGKGSHFKVTAPNGKTTTFADHGAKEMPEPTRKAIIKQLGL

Nucleotide

• Length: 180 bp

>T171951 NZ_CP079116:c14256-13906 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 192 a.a.
• Molecular weight: 21294.78 Da
• Isoelectric Point: 8.3556

>AT171951 WP_021263386.1 NZ_CP061034:c2451365-2450790 [Pseudomonas aeruginosa]
MYNFAIRFEQDDSAPGVAVFCRDLPELNSYGDTVEHALSEAVDAIETTLSLYVDQRRSIPVASAPEPEEHVVHLPAVTVA
KIALWNEMMRRGMRKADLCRVLGIAQVQGDRLVDFLHTSKMEQVESALAALEMRLVICAEPVKQPKQCKGAGCIRDRRLV
KARRLLRARLLELTESSGAFTRAPKIKKPGI

Nucleotide

• Length: 576 bp

>AT171951 NZ_CP079116:c13901-13599 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A844NUK8