Detailed information of TA system TA171949

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 1833523..1835031
• Replicon: chromosome
• Accession: NZ_CP061034
• Organism: Pseudomonas aeruginosa strain PA3

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: EBO27_RS08510
• Protein ID: WP_012613851.1
• Coordinates: 1833523..1834110 (+)
• Length: 196 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: EBO27_RS08515
• Protein ID: WP_012613852.1
• Coordinates: 1834177..1835031 (+)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EBO27_RS08505	1829778..1833257	-	3480	WP_003117605.1	hybrid sensor histidine kinase/response regulator	-
EBO27_RS08510	1833523..1834110	+	588	WP_012613851.1	GNAT family N-acetyltransferase	Toxin
EBO27_RS08515	1834177..1835031	+	855	WP_012613852.1	AraC family transcriptional regulator	Antitoxin
EBO27_RS08520	1835128..1837293	+	2166	WP_003091606.1	TonB-dependent receptor family protein	-
EBO27_RS08525	1837468..1839342	+	1875	WP_187787342.1	MFS transporter	-
EBO27_RS08530	1839403..1839612	-	210	WP_003091604.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (20-152)

Antitoxin

• AraC-type arabinose-binding/dimerisation domain (19-151)

Sequences

Toxin

Protein

• Length: 196 a.a.
• Molecular weight: 21929.29 Da
• Isoelectric Point: 9.8676

>T171949 WP_012613851.1 NZ_CP061034:1833523-1834110 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLAEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAVLEASFTG

Nucleotide

• Length: 588 bp

>T171949 NZ_CP079115:c4934430-4934146 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGCGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31959.27 Da
• Isoelectric Point: 7.3276

>AT171949 WP_012613852.1 NZ_CP061034:1834177-1835031 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHADSLETQTRWRETVLALFRRH
ARLPEAPKAGQEPLPVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTIYE

Nucleotide

• Length: 855 bp

>AT171949 NZ_CP079115:c4934662-4934420 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGTTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA