Detailed information of TA system TA171948

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 143811..144316
• Replicon: chromosome
• Accession: NZ_CP061034
• Organism: Pseudomonas aeruginosa strain PA3

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: EBO27_RS00660
• Protein ID: WP_003083773.1
• Coordinates: 143811..144092 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: EBO27_RS00665
• Protein ID: WP_003083775.1
• Coordinates: 144089..144316 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EBO27_RS00635	139062..140411	+	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-
EBO27_RS00640	140460..141146	+	687	WP_003083762.1	FadR family transcriptional regulator	-
EBO27_RS00645	141247..141981	+	735	WP_003117208.1	GntR family transcriptional regulator	-
EBO27_RS00650	142185..142571	+	387	WP_014602345.1	aegerolysin family protein	-
EBO27_RS00655	142603..143511	-	909	WP_016561475.1	LysR family transcriptional regulator	-
EBO27_RS00660	143811..144092	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
EBO27_RS00665	144089..144316	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
EBO27_RS00670	144492..145112	-	621	WP_003101226.1	hypothetical protein	-
EBO27_RS00675	145213..145713	+	501	WP_003101228.1	LEA type 2 family protein	-
EBO27_RS00680	145786..146127	+	342	WP_003101229.1	alkylphosphonate utilization protein	-
EBO27_RS00685	146209..147636	-	1428	WP_003083784.1	GABA permease	-
EBO27_RS00690	147805..149298	-	1494	WP_003083788.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T171948 WP_003083773.1 NZ_CP061034:c144092-143811 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T171948 NZ_CP079115:c4802439-4801906 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGATTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT171948 WP_003083775.1 NZ_CP061034:c144316-144089 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT171948 NZ_CP079115:c4802716-4802450 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9