Detailed information of TA system TA171944

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 1306171..1306867
• Replicon: chromosome
• Accession: NZ_CP061030
• Organism: Staphylococcus pseudintermedius strain Z0118SP0108

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: A0A828ZQ58
• Locus tag: IAU63_RS06260
• Protein ID: WP_002334977.1
• Coordinates: 1306445..1306867 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: Q9AL01
• Locus tag: IAU63_RS06255
• Protein ID: WP_002331065.1
• Coordinates: 1306171..1306443 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAU63_RS06225	1301470..1302378	+	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
IAU63_RS06230	1302375..1302917	+	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-
IAU63_RS06235	1303010..1303804	+	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
IAU63_RS06240	1304080..1304652	+	573	WP_187470746.1	HTH domain-containing protein	-
IAU63_RS06245	1304950..1305846	+	897	WP_002334978.1	ParA family protein	-
IAU63_RS06250	1305945..1306154	+	210	WP_000527318.1	peptide-binding protein	-
IAU63_RS06255	1306171..1306443	+	273	WP_002331065.1	antitoxin	Antitoxin
IAU63_RS06260	1306445..1306867	+	423	WP_002334977.1	zeta toxin family protein	Toxin
IAU63_RS06265	1306921..1307601	+	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
IAU63_RS06270	1307631..1307783	-	153	WP_029682262.1	hypothetical protein	-
IAU63_RS06275	1307728..1308465	-	738	WP_002292226.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
IAU63_RS06280	1308590..1308673	-	84	WP_001814874.1	23S rRNA methyltransferase attenuation leader peptide	-
IAU63_RS06285	1308722..1308963	-	242	Protein_1209	peptide-binding protein	-
IAU63_RS06290	1309097..1310314	-	1218	Protein_1210	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
IAU63_RS06295	1310830..1311741	+	912	Protein_1211	replication initiation factor domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	blaZ / dfrG / ant(6)-Ia / aph(3')-III / erm(B) / cat(pC221)	-	1212933..1332658	119725
	-	inside	IScluster/Tn	ant(6)-Ia / aph(3')-III / erm(B) / cat(pC221)	-	1297261..1316791	19530

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (28-134)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15667.64 Da
• Isoelectric Point: 5.2420

>T171944 WP_002334977.1 NZ_CP061030:1306445-1306867 [Staphylococcus pseudintermedius]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEALISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSGSVAKF

Nucleotide

• Length: 423 bp

>T171944 NZ_CP079115:2048505-2048607 [Klebsiella pneumoniae subsp. pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10733.17 Da
• Isoelectric Point: 4.4865

>AT171944 WP_002331065.1 NZ_CP061030:1306171-1306443 [Staphylococcus pseudintermedius]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILNKEKVA

Nucleotide

• Length: 273 bp

>AT171944 NZ_CP079115:c2048614-2048469 [Klebsiella pneumoniae subsp. pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Structures

Toxin

• AlphaFold DB: A0A828ZQ58

Antitoxin

• AlphaFold DB: A0A828ZL55