Detailed information of TA system TA171942

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 333055..333576
• Replicon: chromosome
• Accession: NZ_CP061030
• Organism: Staphylococcus pseudintermedius strain Z0118SP0108

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: -
• Locus tag: IAU63_RS01835
• Protein ID: WP_110178839.1
• Coordinates: 333055..333276 (-)
• Length: 74 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: A0A166R1D0
• Locus tag: IAU63_RS01840
• Protein ID: WP_014613363.1
• Coordinates: 333289..333576 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAU63_RS01815	328517..329245	-	729	WP_014613368.1	hypothetical protein	-
IAU63_RS01820	330332..330478	-	147	WP_014613367.1	hypothetical protein	-
IAU63_RS01825	330564..332744	-	2181	WP_187470860.1	heavy metal translocating P-type ATPase	-
IAU63_RS01830	332845..333051	-	207	WP_014613365.1	cation transporter	-
IAU63_RS01835	333055..333276	-	222	WP_110178839.1	heavy-metal-associated domain-containing protein	Toxin
IAU63_RS01840	333289..333576	-	288	WP_014613363.1	copper-sensing transcriptional repressor CsoR	Antitoxin
IAU63_RS01845	333708..335198	+	1491	WP_063284938.1	cardiolipin synthase	-
IAU63_RS01850	335219..335875	+	657	WP_014613361.1	HD domain-containing protein	-
IAU63_RS01855	335929..336795	-	867	WP_014613360.1	membrane protein insertase YidC	-
IAU63_RS01860	336884..337522	-	639	WP_063284943.1	thiamine phosphate synthase	-
IAU63_RS01865	337512..338315	-	804	WP_014613358.1	hydroxyethylthiazole kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (21-64)

Antitoxin

• Metal-sensitive transcriptional repressor (3-93)

Sequences

Toxin

Protein

• Length: 74 a.a.
• Molecular weight: 8081.10 Da
• Isoelectric Point: 4.3480

>T171942 WP_110178839.1 NZ_CP061030:c333276-333055 [Staphylococcus pseudintermedius]
MENGYVKVTQLTTEAQQQQLSDRLLRLIGVESVAIDVATQSLTLTYDTPANLNTIEKEIYDAGYPVIQSHKGV

Nucleotide

• Length: 222 bp

>T171942 NZ_CP079115:335831-336199 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10997.59 Da
• Isoelectric Point: 7.0504

>AT171942 WP_014613363.1 NZ_CP061030:c333576-333289 [Staphylococcus pseudintermedius]
MTEQAHHSEEIKRNLKSRLNRIEGQVKAINRMIDEDVYCDDVLTQIRATRSALNSVASRLLDHHMKGCIKHKIDAGHQDE
AMEELLVTFQKLMKD

Nucleotide

• Length: 288 bp

>AT171942 NZ_CP079115:335613-335834 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A166R1D0