Detailed information of TA system TA171931

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PHD(antitoxin)
• Location: 5453996..5454669
• Replicon: chromosome
• Accession: NZ_CP061007
• Organism: Saccharopolyspora spinosa strain CCTCC M206084

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A2N3XX88
• Locus tag: IAG28_RS24605
• Protein ID: WP_010695010.1
• Coordinates: 5454268..5454669 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: IAG28_RS24600
• Protein ID: WP_029535249.1
• Coordinates: 5453996..5454268 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAG28_RS24580	5449284..5449787	-	504	WP_010695015.1	YbaB/EbfC family nucleoid-associated protein	-
IAG28_RS24585	5450018..5451961	+	1944	WP_010695014.1	1-deoxy-D-xylulose-5-phosphate synthase	-
IAG28_RS24590	5452118..5452804	+	687	WP_029535251.1	response regulator transcription factor	-
IAG28_RS24595	5452809..5453954	+	1146	WP_010695012.1	HAMP domain-containing histidine kinase	-
IAG28_RS24600	5453996..5454268	+	273	WP_029535249.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
IAG28_RS24605	5454268..5454669	+	402	WP_010695010.1	type II toxin-antitoxin system VapC family toxin	Toxin
IAG28_RS24610	5454950..5457100	-	2151	WP_010695009.1	enoyl-CoA hydratase/isomerase family protein	-
IAG28_RS24615	5457097..5458338	-	1242	WP_010695008.1	thiolase family protein	-
IAG28_RS24620	5458510..5459232	+	723	WP_010695007.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14518.69 Da
• Isoelectric Point: 5.5280

>T171931 WP_010695010.1 NZ_CP061007:5454268-5454669 [Saccharopolyspora spinosa]
VIYLDSAALIKLVRRERESDALADWLEQQPNVPLVASALVEVEVPRALRRYEPNLLSAVPAVLKRIGVYDIDDVVRATAA
AYQDVLLCSIDAIHLATAHAIFGSHLTAFVTYDHRLHKAAEDVGLPVAQPGTV

Nucleotide

• Length: 402 bp

>T171931 NZ_CP079097:1820583-1820927 [Methylococcus capsulatus]
GTGACCTATGAGCGCGGCCAAATCGTGCGCGTGCCCTTCCCCTTCACCGACCGCGCCGCCAGCAAGAACCGCCCGGCGCT
GGTGCTCTCCGATGCCAGCGCCTTCAACACCCCCGCCGGGCACGTGGTGCTGGCCATGATCACCTCGGCCAAGAACCCGT
CCTGGCCGCTGGACTGCGCCATCGAAGACCTGGCCGCCGCCGGCCTGCCCGCCCCTTCGGTGGTGCGCTGCAAGCTGTTC
ACCCTGGATGCCCGGCTCATCCGCGGGGTGCTCGGCGTACTGGCTGAAACCGACGCGCAGCGCACGGCGCAGACCCTCGC
CCAACTGCTCGGCTCCTACTCATGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9922.47 Da
• Isoelectric Point: 7.2054

>AT171931 WP_029535249.1 NZ_CP061007:5453996-5454268 [Saccharopolyspora spinosa]
MSEVPVRMLNQETSSVLARVKRGEEIDITERGAIIARIIPAHPHPLGELIASGKFRPPTLEGSMPRPLGPVRTDVDAGAL
IRELRDAERY

Nucleotide

• Length: 273 bp

>AT171931 NZ_CP079097:1820363-1820593 [Methylococcus capsulatus]
ATGACCGCCGTGGCCAAAATCACCGCCAAGGGCCAGACCACCATCCCCGCCGAGATCCGCGCCGCGCTCCACGTCAGCCC
CGGCGACCTCATCGCCTGGGAGATCGAACCCGACGGCAGCGCGCGCGTGCGCCGTGTGCAACCGCTCGACATCGAATACC
TGAAGGCCGTCGAGGGCACCCTGTCCGAGTGGACGAGCCCGGCCGACGAGGAGGCCTACCGTGACCTATGA

Structures

Toxin

• AlphaFold DB: A0A2N3XX88