Detailed information of TA system TA171930

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 4860889..4861453
• Replicon: chromosome
• Accession: NZ_CP061007
• Organism: Saccharopolyspora spinosa strain CCTCC M206084

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A2N3XVP7
• Locus tag: IAG28_RS21770
• Protein ID: WP_010696020.1
• Coordinates: 4860889..4861236 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: IAG28_RS21775
• Protein ID: WP_010696019.1
• Coordinates: 4861223..4861453 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAG28_RS21750	4856553..4857839	+	1287	WP_101377220.1	lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family protein	-
IAG28_RS21755	4858004..4859098	+	1095	WP_010696023.1	LLM class flavin-dependent oxidoreductase	-
IAG28_RS21760	4859387..4859785	+	399	WP_010696022.1	DUF3817 domain-containing protein	-
IAG28_RS21765	4859782..4860717	+	936	WP_010696021.1	heme o synthase	-
IAG28_RS21770	4860889..4861236	-	348	WP_010696020.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IAG28_RS21775	4861223..4861453	-	231	WP_010696019.1	ribbon-helix-helix domain-containing protein	Antitoxin
IAG28_RS21780	4861577..4862200	-	624	WP_010696017.1	maleylpyruvate isomerase N-terminal domain-containing protein	-
IAG28_RS21785	4862197..4862931	-	735	WP_010696015.1	PIG-L family deacetylase	-
IAG28_RS21790	4863279..4863647	+	369	WP_010696013.1	snapalysin family zinc-dependent metalloprotease	-
IAG28_RS21795	4863927..4864778	+	852	WP_010696010.1	nucleotide exchange factor GrpE	-
IAG28_RS21800	4864771..4866426	+	1656	WP_010696009.1	Hsp70 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-114)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12607.46 Da
• Isoelectric Point: 11.5692

>T171930 WP_010696020.1 NZ_CP061007:c4861236-4860889 [Saccharopolyspora spinosa]
MRRGDLYWVDFEPIRGSEANKTRPAVVISNDAANRSAQRAGRGVITVVPVTSNIDRVHPFQVLLRAAECGLPNDSKAQAE
QVRAVAVNRLGNRIGTLPQSTLAKLESALRLHLRL

Nucleotide

• Length: 348 bp

>T171930 NZ_CP079095:c2625382-2625050 [Methylococcus sp. Mc7]
ATGGCTGAAGTAATCTGGACCGAACCAGCTCTCGGTGATCTGGATGCCATTGCCGATTACATTGCCTTAGACAACCCAGA
AGCGGCGCGAAGCCTCGTCCAGAGAGTTTTTGAGCATGTTGATCATCTGGAAAGACATCCTCAGCTCGGCTCGAAGCCGC
CGGAACTCAAGGGGTGGGGTTGCCGTCAGATCGTGGAACCACCCTGCCGAATCTTCTATCGCGAGGATTCGGGCCATGTG
TTCATTCTTCATGTCATGCGGGCCGAGCGCCTTCTGAAGCCCGAACTGCTTGAGACTCGAGATAAGGAAACGGGTGGGAC
AAACCCGTTTTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8165.86 Da
• Isoelectric Point: 3.7671

>AT171930 WP_010696019.1 NZ_CP061007:c4861453-4861223 [Saccharopolyspora spinosa]
MKVSVSLTDEDVTFVDEYAQQIGAPSRSAVIHQAINLLRNAGLEDAYASAWEEWDGSADAQLWDATAADGIADAPR

Nucleotide

• Length: 231 bp

>AT171930 NZ_CP079095:c2625627-2625370 [Methylococcus sp. Mc7]
ATGCGTACTGAACTCGTGACCACTCTCAAGCGCCAAGCCACCGAGCTGCTATCCCGGCTAGCCCGTGATCGTGAACCGAT
CCTGATCACGCAACATGGCGTGCCGGCGGCGTATCTGGTGGATGTTGAAACTTTCGAGACTCTTCAACGGCGGATGGTTC
TACTCGAGGGCATTGCGCGAGGCGAGAGAGCCATTGAGGAAGGACGTACCCTTACCCAGGCCGAGGCGAAGCAACGCATG
GCCAGATGGCTGAAGTAA

Structures

Toxin

• AlphaFold DB: A0A2N3XVP7