Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PHD(antitoxin)
Location 2047967..2048631 Replicon chromosome
Accession NZ_CP061007
Organism Saccharopolyspora spinosa strain CCTCC M206084

Toxin (Protein)


Gene name vapC Uniprot ID A0A2N3Y9L9
Locus tag IAG28_RS08095 Protein ID WP_010306093.1
Coordinates 2047967..2048362 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A2N3Y9G1
Locus tag IAG28_RS08100 Protein ID WP_010306091.1
Coordinates 2048359..2048631 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IAG28_RS08075 2044044..2044763 + 720 WP_203218584.1 FadR family transcriptional regulator -
IAG28_RS08080 2044788..2045036 - 249 WP_010306100.1 type II toxin-antitoxin system prevent-host-death family antitoxin -
IAG28_RS08085 2045145..2046665 - 1521 WP_101377091.1 cobyric acid synthase -
IAG28_RS08090 2046971..2047828 - 858 WP_010306096.1 type I methionyl aminopeptidase -
IAG28_RS08095 2047967..2048362 - 396 WP_010306093.1 type II toxin-antitoxin system VapC family toxin Toxin
IAG28_RS08100 2048359..2048631 - 273 WP_010306091.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
IAG28_RS08105 2048689..2050476 - 1788 WP_044573203.1 penicillin-binding protein -
IAG28_RS08110 2050653..2051489 - 837 WP_187764702.1 GNAT family N-acetyltransferase -
IAG28_RS08115 2051683..2052837 - 1155 WP_010306079.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-114)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-41)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14203.30 Da        Isoelectric Point: 5.5837

>T171928 WP_010306093.1 NZ_CP061007:c2048362-2047967 [Saccharopolyspora spinosa]
MIYFDSSALIKLIAPEPESKALSQWIRERWEHPRVTSALSKVDVLRTFREVSPAAEDLASIIVSKIDQLPVKQDVLDVAT
ELRCNVGPVDAIHLASACKIKHGLHAYVSYDPALLAAAEDAGLTTASPGVN

Download         Length: 396 bp

>T171928 NZ_CP079095:c2467342-2466845 [Methylococcus sp. Mc7]
TTGCCGCTTCTGGCTCCCCGACCGCTCGCTGAACACCATCGAACGGAAAGCTTCGCTTCCGGCATTACCTCCCTGGACGA
CTGGTTGAAGCGCAGGGCATTGGCCAATCAGTCCAGTGGCGCCTCCCGGACCTTCGTGGTCTGCGACGAACACCGGGTGA
TCGGGTATTACGCCCTCGCGGCCGGAGCGATCAGAATCCAGGACGCTTCGGGCCGGTTTCGGCGCAATATGCCCGATCCC
ATTCCCGTCGCGATTCTGGCGCGTCTCGCCGTGGATCAAGCTTACCAGGGTCAAGGGATTGGCCGCGCCCTATTCCGCGA
CGGCGCCCGACGTGTGGTTCAGGCTGCGGATACGCTTGGCATTCGTGGGATCGTCGTCCACGCCATTTCCGAGGACGCCA
AGGCGTTTTATCAAGCGCTGGGCTTTGATCCATCCCCGGTTGATGCCATGACACTCCTGGTGACGTTAAGCGACCTGCGC
GCCGCACTGCCCGGCTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9974.34 Da        Isoelectric Point: 6.3673

>AT171928 WP_010306091.1 NZ_CP061007:c2048631-2048359 [Saccharopolyspora spinosa]
MERIGVRELRQNASRYLKRVAAGESILVTDRGRTVAVLSPPTRDQTLYDELVAAGELVPGEGGELPEPLPAAPTPNVSDR
LLRRREQERL

Download         Length: 273 bp

>AT171928 NZ_CP079095:c2467630-2467346 [Methylococcus sp. Mc7]
ATGACCACGACTCACTCTCAGACCACCAGGCGCGATAGCCTGAATATCCGCATCAAACCGGAAGATCGCGGATTGATTGA
CCGGGCCGCCAAGCTTTTGGGCAAGAACCGGACGGATTTCATGCTCGATGCCGCGCGTAGAGCCGCCGAGGAGGCTCTAC
TCGACCGCGCCGTGTTCGTGGTGAGTCCCGAAGCCTATGCCGAATTTCTCGCGCGTCTGGATGCGCCGCCGCAGCCCAAC
GAGCGCCTGCGAAAAACCATGCAGACGTCCGCGCCGTGGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N3Y9L9

Antitoxin

Source ID Structure
AlphaFold DB A0A2N3Y9G1

References