Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4468306..4468803 Replicon chromosome
Accession NZ_CP060987
Organism Escherichia coli strain EC31

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag H9212_RS21770 Protein ID WP_001173343.1
Coordinates 4468306..4468479 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag H9212_RS21775 Protein ID WP_000398619.1
Coordinates 4468507..4468803 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9212_RS21760 (4464976) 4464976..4466433 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
H9212_RS21765 (4466670) 4466670..4468187 + 1518 WP_001295074.1 lysine--tRNA ligase -
H9212_RS21770 (4468306) 4468306..4468479 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
H9212_RS21775 (4468507) 4468507..4468803 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
H9212_RS21780 (4469030) 4469030..4469302 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
H9212_RS21785 (4469314) 4469314..4469544 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
H9212_RS21790 (4469725) 4469725..4471356 + 1632 WP_001216477.1 sensor histidine kinase -
H9212_RS21795 (4471353) 4471353..4472072 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T171914 WP_001173343.1 NZ_CP060987:c4468479-4468306 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T171914 NZ_CP078521:c2011181-2010930 [Staphylococcus aureus]
ATGAATAATCGCGAACAAATTGAACAATCAGTTATAAGTGCTAGTGCGTATAACGGTAATGACACAGAGGGATTACTAAA
AGAGATTGAGGACGTTTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGAGGGAATGACAAATGCTATTCAACATTCAG
TTAAAGAAGGTGTTGAACTTGATGAAGCAGTAGGGATTATGGCAGGTCAAGTTGTCTATAAATATGAGGAGGAACAGGAA
AATGAGCATTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT171914 WP_000398619.1 NZ_CP060987:c4468803-4468507 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT171914 NZ_CP078521:c2010940-2010758 [Staphylococcus aureus]
ATGAGCATTAGTGTAGGAGACAAGGTTTTTAATCCAGAAACAAATTCAACTTTAGAAATTGTACAACTTGTTGGCGATAT
TAGAGACACGCATTACAAGTTATCTGACGGATCTATTATTAGTCTTATAGACTTTGTTGTTAAACCAATTCATTTAATCA
AGGAGGAGCAGGAAAATGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References