Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 932562..933216 Replicon chromosome
Accession NZ_CP060987
Organism Escherichia coli strain EC31

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag H9212_RS04585 Protein ID WP_000244777.1
Coordinates 932809..933216 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag H9212_RS04580 Protein ID WP_000354046.1
Coordinates 932562..932828 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9212_RS04555 (927731) 927731..928474 + 744 WP_000951964.1 SDR family oxidoreductase -
H9212_RS04560 (928531) 928531..929964 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
H9212_RS04565 (930009) 930009..930320 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
H9212_RS04570 (930484) 930484..931143 + 660 WP_000250274.1 hemolysin III family protein -
H9212_RS04575 (931339) 931339..932319 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
H9212_RS04580 (932562) 932562..932828 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
H9212_RS04585 (932809) 932809..933216 + 408 WP_000244777.1 protein YgfX Toxin
H9212_RS04590 (933256) 933256..933777 - 522 WP_001055874.1 flavodoxin FldB -
H9212_RS04595 (933889) 933889..934785 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
H9212_RS04600 (934810) 934810..935520 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
H9212_RS04605 (935526) 935526..937259 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T171895 WP_000244777.1 NZ_CP060987:932809-933216 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T171895 NZ_CP078162:2561598-2561738 [Enterococcus faecalis]
ATTTCTCTGAAAAATACGAATAAAAATATCGTTTACTGTACAACATATGAAACAATTCAGACGATCCTAGGGTTTGGTAT
GTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT171895 WP_000354046.1 NZ_CP060987:932562-932828 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT171895 NZ_CP078162:c2561872-2561671 [Enterococcus faecalis]
GTTTTTTTAGGTAATTGTGCTATAACAGCAATGAAAAGAGAGATATGCGCCAACATACCTCTCTGATGTAGAGCCGTTTA
AGACGGTGACCTTTTTGATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCA
ATTTCACAATCAGCGCAATCAAAGCAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References