Detailed information of TA system TA171894

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 796747..797579
• Replicon: chromosome
• Accession: NZ_CP060987
• Organism: Escherichia coli strain EC31

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: H9212_RS03880
• Protein ID: WP_000854765.1
• Coordinates: 796747..797121 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: H9212_RS03885
• Protein ID: WP_001295723.1
• Coordinates: 797211..797579 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9212_RS03845 (791825)	791825..792424	+	600	WP_001255034.1	type II secretion system minor pseudopilin GspJ	-
H9212_RS03850 (792427)	792427..793404	+	978	WP_000633209.1	type II secretion system minor pseudopilin GspK	-
H9212_RS03855 (793401)	793401..794579	+	1179	WP_000094991.1	type II secretion system protein GspL	-
H9212_RS03860 (794581)	794581..795117	+	537	WP_000942785.1	GspM family type II secretion system protein YghD	-
H9212_RS03865 (795398)	795398..796240	-	843	WP_022645115.1	DUF4942 domain-containing protein	-
H9212_RS03870 (796325)	796325..796519	-	195	WP_024181941.1	DUF957 domain-containing protein	-
H9212_RS03875 (796544)	796544..796747	-	204	WP_001398797.1	DUF5983 family protein	-
H9212_RS03880 (796747)	796747..797121	-	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
H9212_RS03885 (797211)	797211..797579	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
H9212_RS03890 (797742)	797742..797963	-	222	WP_000692341.1	DUF987 domain-containing protein	-
H9212_RS03895 (798026)	798026..798502	-	477	WP_001186774.1	RadC family protein	-
H9212_RS03900 (798518)	798518..798997	-	480	WP_057109541.1	antirestriction protein	-
H9212_RS03905 (799263)	799263..800081	-	819	WP_001175165.1	DUF932 domain-containing protein	-
H9212_RS03910 (800171)	800171..800404	-	234	WP_001278293.1	DUF905 family protein	-
H9212_RS03915 (800410)	800410..801087	-	678	WP_001097302.1	hypothetical protein	-
H9212_RS03920 (801235)	801235..801915	-	681	WP_001282921.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T171894 WP_000854765.1 NZ_CP060987:c797121-796747 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T171894 NZ_CP078162:2561598-2561738 [Enterococcus faecalis]
ATTTCTCTGAAAAATACGAATAAAAATATCGTTTACTGTACAACATATGAAACAATTCAGACGATCCTAGGGTTTGGTAT
GTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT171894 WP_001295723.1 NZ_CP060987:c797579-797211 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT171894 NZ_CP078162:c2561596-2561453 [Enterococcus faecalis]
TGCTACAATAGAGACGAAAAGAGAGGTATGCTCTAACATACCTCTCTAGTGTAGAGCCGTTTAAGACGGTGACCTTTTTA
GTTACAAAAAATAACCGTACTCAGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52