Detailed information of TA system TA171890

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 7110..7702
• Replicon: plasmid pEC3-5
• Accession: NZ_CP060983
• Organism: Escherichia coli strain EC3

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: V0AGQ2
• Locus tag: H9191_RS24630
• Protein ID: WP_000520549.1
• Coordinates: 7110..7358 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V0AWG0
• Locus tag: H9191_RS24635
• Protein ID: WP_000681613.1
• Coordinates: 7355..7702 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9191_RS24585 (H9191_24365)	2447..2698	+	252	WP_000121743.1	plasmid stabilization protein	-
H9191_RS24590 (H9191_24370)	2688..2969	+	282	WP_000220561.1	type II toxin-antitoxin system RelE/ParE family toxin	-
H9191_RS24595 (H9191_24375)	2993..3343	+	351	WP_000312755.1	hypothetical protein	-
H9191_RS24600 (H9191_24380)	3983..4498	+	516	WP_001025397.1	J domain-containing protein	-
H9191_RS24605 (H9191_24385)	4603..5145	+	543	WP_001083906.1	hypothetical protein	-
H9191_RS24610 (H9191_24390)	5142..5393	+	252	WP_001230707.1	hypothetical protein	-
H9191_RS24615 (H9191_24395)	5380..5607	+	228	WP_000801440.1	hypothetical protein	-
H9191_RS24620 (H9191_24400)	6061..6360	+	300	WP_000650310.1	hypothetical protein	-
H9191_RS24625 (H9191_24405)	6455..6805	+	351	WP_000854260.1	hypothetical protein	-
H9191_RS24630 (H9191_24410)	7110..7358	+	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	Toxin
H9191_RS24635 (H9191_24415)	7355..7702	+	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
H9191_RS24640 (H9191_24420)	8066..8569	+	504	WP_000781817.1	DNA distortion polypeptide 1	-
H9191_RS24645 (H9191_24425)	8573..9802	+	1230	WP_001329504.1	DNA relaxase/nickase TaxC	-
H9191_RS24650 (H9191_24430)	9879..10145	+	267	WP_000160396.1	hypothetical protein	-
H9191_RS24655 (H9191_24435)	10150..10383	+	234	WP_000121161.1	EexN family lipoprotein	-
H9191_RS24660 (H9191_24440)	10739..11359	+	621	WP_000157548.1	lytic transglycosylase domain-containing protein	-
H9191_RS24665 (H9191_24445)	11373..11699	+	327	WP_000008647.1	TrbC/VirB2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..35307	35307

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9422.08 Da
• Isoelectric Point: 10.4800

>T171890 WP_000520549.1 NZ_CP060983:7110-7358 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Nucleotide

• Length: 249 bp

>T171890 NZ_CP078162:c2484303-2484199 [Enterococcus faecalis]
TTGTCAGCATATGAAACAATTCAGACGCTCCTAGGGTTTGGTATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATT
GCTTAAAAATGACAATAAAAAATAA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13113.84 Da
• Isoelectric Point: 4.6723

>AT171890 WP_000681613.1 NZ_CP060983:7355-7702 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Nucleotide

• Length: 348 bp

>AT171890 NZ_CP078162:2484080-2484266 [Enterococcus faecalis]
TGTGCTATAATGAAAACGAAAAGAGAGATATGCGTCAACATACCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCGATT
TTGTTACAAAAAATAACCGTACTCGATCAAAGTAAACGGTTATTTTTTATTGTCATTTTTAAGCAATTTCACAATCAGCG
CAATCAAAGCAATGGTAAACATACCAA

Structures

Toxin

• AlphaFold DB: A0A829CIX3

Antitoxin

• AlphaFold DB: A0A829CLC1