171889

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 5142..5607 Replicon plasmid pEC3-5
Accession NZ_CP060983
Organism Escherichia coli strain EC3

Toxin (Protein)


Gene name TsxA Uniprot ID U9ZUY2
Locus tag H9191_RS24610 Protein ID WP_001230707.1
Coordinates 5142..5393 (+) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag H9191_RS24615 Protein ID WP_000801440.1
Coordinates 5380..5607 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9191_RS24585 (H9191_24365) 2447..2698 + 252 WP_000121743.1 plasmid stabilization protein -
H9191_RS24590 (H9191_24370) 2688..2969 + 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin -
H9191_RS24595 (H9191_24375) 2993..3343 + 351 WP_000312755.1 hypothetical protein -
H9191_RS24600 (H9191_24380) 3983..4498 + 516 WP_001025397.1 J domain-containing protein -
H9191_RS24605 (H9191_24385) 4603..5145 + 543 WP_001083906.1 hypothetical protein -
H9191_RS24610 (H9191_24390) 5142..5393 + 252 WP_001230707.1 hypothetical protein Toxin
H9191_RS24615 (H9191_24395) 5380..5607 + 228 WP_000801440.1 hypothetical protein Antitoxin
H9191_RS24620 (H9191_24400) 6061..6360 + 300 WP_000650310.1 hypothetical protein -
H9191_RS24625 (H9191_24405) 6455..6805 + 351 WP_000854260.1 hypothetical protein -
H9191_RS24630 (H9191_24410) 7110..7358 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin -
H9191_RS24635 (H9191_24415) 7355..7702 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin -
H9191_RS24640 (H9191_24420) 8066..8569 + 504 WP_000781817.1 DNA distortion polypeptide 1 -
H9191_RS24645 (H9191_24425) 8573..9802 + 1230 WP_001329504.1 DNA relaxase/nickase TaxC -
H9191_RS24650 (H9191_24430) 9879..10145 + 267 WP_000160396.1 hypothetical protein -
H9191_RS24655 (H9191_24435) 10150..10383 + 234 WP_000121161.1 EexN family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..35307 35307


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Small toxic protein BsrE-like

(1-33)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9751.13 Da        Isoelectric Point: 8.9187

>T171889 WP_001230707.1 NZ_CP060983:5142-5393 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T171889 NZ_CP078162:c2411766-2411665 [Enterococcus faecalis]
TTGTCAATCGAAGCGGCATTGGAATTGATGATTAGTTTTGCAGCGTTTGTTGCACTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACAATAAAAAATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT171889 WP_000801440.1 NZ_CP060983:5380-5607 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT171889 NZ_CP078162:2411546-2411720 [Enterococcus faecalis]
AAAAGAGAGGTATGCGGGTACATACCTCTCCTTTTATACCAACACCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGTT
TTGAATCTTAACCGTTCGGCCTACGAAGCTTGTGAACGGTTATTTTTTATTGTTTTTCGTTGCTTCAAGGATACCGAAAA
TCAGTAGTGCAACAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9ZUY2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References