Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4198080..4198577 Replicon chromosome
Accession NZ_CP060979
Organism Escherichia coli strain EC3

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag H9191_RS20655 Protein ID WP_001173343.1
Coordinates 4198080..4198253 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag H9191_RS20660 Protein ID WP_000398619.1
Coordinates 4198281..4198577 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9191_RS20645 (4194750) 4194750..4196207 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
H9191_RS20650 (4196444) 4196444..4197961 + 1518 WP_001295074.1 lysine--tRNA ligase -
H9191_RS20655 (4198080) 4198080..4198253 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
H9191_RS20660 (4198281) 4198281..4198577 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
H9191_RS20665 (4198804) 4198804..4199076 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
H9191_RS20670 (4199088) 4199088..4199318 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
H9191_RS20675 (4199499) 4199499..4201130 + 1632 WP_001216487.1 sensor histidine kinase -
H9191_RS20680 (4201127) 4201127..4201846 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T171874 WP_001173343.1 NZ_CP060979:c4198253-4198080 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T171874 NZ_CP078146:c3445956-3445854 [Klebsiella variicola]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT171874 WP_000398619.1 NZ_CP060979:c4198577-4198281 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT171874 NZ_CP078146:3445848-3445992 [Klebsiella variicola]
ACCGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References