Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3453449..3454067 Replicon chromosome
Accession NZ_CP060979
Organism Escherichia coli strain EC3

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H9191_RS17035 Protein ID WP_001291435.1
Coordinates 3453849..3454067 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H9191_RS17030 Protein ID WP_000344800.1
Coordinates 3453449..3453823 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9191_RS17020 (3448538) 3448538..3449731 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H9191_RS17025 (3449754) 3449754..3452903 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
H9191_RS17030 (3453449) 3453449..3453823 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H9191_RS17035 (3453849) 3453849..3454067 + 219 WP_001291435.1 HHA domain-containing protein Toxin
H9191_RS17040 (3454239) 3454239..3454790 + 552 WP_000102564.1 maltose O-acetyltransferase -
H9191_RS17045 (3454906) 3454906..3455376 + 471 WP_000136192.1 YlaC family protein -
H9191_RS17050 (3455540) 3455540..3457090 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H9191_RS17055 (3457132) 3457132..3457485 - 354 WP_000878140.1 DUF1428 domain-containing protein -
H9191_RS17065 (3457864) 3457864..3458175 + 312 WP_000409911.1 MGMT family protein -
H9191_RS17070 (3458206) 3458206..3458778 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T171870 WP_001291435.1 NZ_CP060979:3453849-3454067 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T171870 NZ_CP078145:7597840-7598232 [Nocardia iowensis]
ATGATCGTGGATGCGTCCGCTCTGCTCGCCATCCTCAACGAGGAACCCGAATCCGACTCGTTCACCGAAATCCTGGGGTC
GGCGCCACGTGTCGCGATGTCGGTCGTCAACCATTTCGAGGTAGCCATCAAGGTCGACCGTCATCCGAATCCGGTGCTCG
CGCGACGCGCTGACCAATTGATGGAGCGAGCACAGGTCGAGCTCGTTCCCGTCACGCTCGAGCAGGCCGCGATCGCGCGA
GCGGCGTATCGCGACTTCGGCAAAGGCAGTGGGCATAAAGCCGAGCTCAACCTCGGCGACTGCTTCGCCTACGCACTCGC
CACCGTCACCAAGAAACCGCTGCTCTACAAGGGCAACGACTTCGGCCACACCGACATCAGATCGGCGGTGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT171870 WP_000344800.1 NZ_CP060979:3453449-3453823 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT171870 NZ_CP078145:7597592-7597843 [Nocardia iowensis]
ATGGGACTGAACATCAAGAATGAGAACGTGCACGCCTTAGTGCGTGAGGCAGCGGAGCGAACGGGGATGTCGCAAACCAG
CGTTGTCGAGGAGGCACTGAAGCGCATGTTGAAAGAACTGGATGCCGAGCAGGAGACTCGGCTAGAAGACCTGCGGGCGA
TCGTGGCGGATATCCACGCGATCATGAGGGAGTCCGAGTACCCGCCCCTGACCACCGACGATCTCTACGATGATGAGGGG
CTGCCCGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References