Detailed information of TA system TA171851

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 6415..7007
• Replicon: plasmid pEC4-4
• Accession: NZ_CP060977
• Organism: Escherichia coli strain EC4

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: V0AGQ2
• Locus tag: H9192_RS24575
• Protein ID: WP_000520549.1
• Coordinates: 6415..6663 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V0AWG0
• Locus tag: H9192_RS24580
• Protein ID: WP_000681613.1
• Coordinates: 6660..7007 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9192_RS24545 (H9192_24260)	3292..3807	+	516	WP_001025395.1	J domain-containing protein	-
H9192_RS24550 (H9192_24265)	3912..4454	+	543	WP_241461987.1	hypothetical protein	-
H9192_RS24555 (H9192_24270)	4451..4702	+	252	WP_001230707.1	hypothetical protein	-
H9192_RS24560 (H9192_24275)	4689..4916	+	228	WP_000801440.1	hypothetical protein	-
H9192_RS24565 (H9192_24280)	5370..5669	+	300	WP_000650309.1	hypothetical protein	-
H9192_RS24570 (H9192_24285)	5760..6110	+	351	WP_000854259.1	hypothetical protein	-
H9192_RS24575 (H9192_24290)	6415..6663	+	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	Toxin
H9192_RS24580 (H9192_24295)	6660..7007	+	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
H9192_RS24585 (H9192_24300)	7368..7871	+	504	WP_000781813.1	relaxosome-coupling protein TaxA	-
H9192_RS24590 (H9192_24305)	7875..9104	+	1230	WP_001329504.1	DNA relaxase/nickase TaxC	-
H9192_RS24595 (H9192_24310)	9181..9474	+	294	WP_094320505.1	sigma factor-like helix-turn-helix DNA-binding protein	-
H9192_RS24600 (H9192_24315)	9471..9686	+	216	WP_000760375.1	EexN family lipoprotein	-
H9192_RS24605 (H9192_24320)	10042..10662	+	621	WP_000157549.1	lytic transglycosylase domain-containing protein	-
H9192_RS24610 (H9192_24325)	10676..11002	+	327	WP_000008647.1	TrbC/VirB2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..31251	31251

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9422.08 Da
• Isoelectric Point: 10.4800

>T171851 WP_000520549.1 NZ_CP060977:6415-6663 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Nucleotide

• Length: 249 bp

>T171851 NZ_CP078139:c5541526-5541176 [Pseudomonas savastanoi pv. savastanoi]
ATGAATACGTTTGCACTGCGCTTTACCGATGTTGCCCAGCAAAGCCTTGAGGATCAGGTTGAACACCTGGCTGTCCATCA
GGGATTCTCATCAGCTGCTCAACGCATAGATACCCTTATCGATGCCATCCAAGACAAGCTCCTGTCTACCCCGTTGGGCT
ACCCGGTCAGCCCTCAATTGAGTGAGCTGGGTGTACTGCACTATCGTGAGCTGAACACTGACGGATACCGGATCTTTTAC
GAGGTGATGGATTCAGATGGTATTACCTTCATCGTCATATTGCTCGTATTAGGAGGTAAACAAAGTGTTGAACAAGCCCT
CATCCGCTATTGCCTTCTTCAGCCTATATAG

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13113.84 Da
• Isoelectric Point: 4.6723

>AT171851 WP_000681613.1 NZ_CP060977:6660-7007 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Nucleotide

• Length: 348 bp

>AT171851 NZ_CP078139:c5541787-5541539 [Pseudomonas savastanoi pv. savastanoi]
ATGCGAGTTGAAACAATCAGTTTTCTGAAACGCAACGCAGCCGACCTGCCTCTCGATGAGCCATTGGTCGTCACGCAAAA
TGGTGTCCCGGCCTACGTGGTGGAGTCTTACGCTGATCGTAAGCGCCGCGATGAGTCAATCGCCTTGGTGAAGCTCCTGG
CAATCAGCTCCCGCGAGTACTCCCAAGGCAAGCACTGTTCCACTGACGAACTCAAAGCCCGATTGAGCAGGAGGTTTACC
CAGAAATAA

Structures

Toxin

• AlphaFold DB: A0A829CIX3

Antitoxin

• AlphaFold DB: A0A829CLC1