Detailed information of TA system TA171850

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 4451..4916
• Replicon: plasmid pEC4-4
• Accession: NZ_CP060977
• Organism: Escherichia coli strain EC4

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9ZUY2
• Locus tag: H9192_RS24555
• Protein ID: WP_001230707.1
• Coordinates: 4451..4702 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: M7CCF8
• Locus tag: H9192_RS24560
• Protein ID: WP_000801440.1
• Coordinates: 4689..4916 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9192_RS24535 (H9192_24250)	594..998	-	405	WP_012421203.1	hypothetical protein	-
H9192_RS24540 (H9192_24255)	1011..1877	-	867	WP_001353743.1	replication initiation protein	-
H9192_RS24545 (H9192_24260)	3292..3807	+	516	WP_001025395.1	J domain-containing protein	-
H9192_RS24550 (H9192_24265)	3912..4454	+	543	WP_241461987.1	hypothetical protein	-
H9192_RS24555 (H9192_24270)	4451..4702	+	252	WP_001230707.1	hypothetical protein	Toxin
H9192_RS24560 (H9192_24275)	4689..4916	+	228	WP_000801440.1	hypothetical protein	Antitoxin
H9192_RS24565 (H9192_24280)	5370..5669	+	300	WP_000650309.1	hypothetical protein	-
H9192_RS24570 (H9192_24285)	5760..6110	+	351	WP_000854259.1	hypothetical protein	-
H9192_RS24575 (H9192_24290)	6415..6663	+	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	-
H9192_RS24580 (H9192_24295)	6660..7007	+	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	-
H9192_RS24585 (H9192_24300)	7368..7871	+	504	WP_000781813.1	relaxosome-coupling protein TaxA	-
H9192_RS24590 (H9192_24305)	7875..9104	+	1230	WP_001329504.1	DNA relaxase/nickase TaxC	-
H9192_RS24595 (H9192_24310)	9181..9474	+	294	WP_094320505.1	sigma factor-like helix-turn-helix DNA-binding protein	-
H9192_RS24600 (H9192_24315)	9471..9686	+	216	WP_000760375.1	EexN family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..31251	31251

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Arc-like DNA binding domain (5-46)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9751.13 Da
• Isoelectric Point: 8.9187

>T171850 WP_001230707.1 NZ_CP060977:4451-4702 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Nucleotide

• Length: 252 bp

>T171850 NZ_CP078139:c4194917-4194498 [Pseudomonas savastanoi pv. savastanoi]
ATGATCCTGCTCGATACGAACGTAATTTCAGAACCGCAGCGCCGAGAGCCCAATGCCCACGTCCTTGACTGGATAGACGC
ACAAGCGCTGGAAACGCTTTATGTGTCCACCATCACGGTAGCCGAGTTGCGTGCAGGTATCGCGCTGATGCCTGTCGGAA
AACGCCAGGATAGCTTGCGTGAAAACCTGGAAAAGCACCTGCTGCCGATGTTCGCCAATAGGGTGCTGTCGTTCGATATG
ACTTGTACGAAGGCTTATGCCGAGCTGCTGGCCAAGTCCCGCGCAGCCGGGTTGGCGGTCGAAACGGCAGATGCCTTCAT
TGCAGCCATCGCCATCGCCAACGGCTTCACCGTCGCCACCCGTGACACTGGCCCTTTTGAGGCTGCCGGGTTGAACGTCA
TTAACCCCTGGGAAGCGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8659.98 Da
• Isoelectric Point: 8.5105

>AT171850 WP_000801440.1 NZ_CP060977:4689-4916 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Nucleotide

• Length: 228 bp

>AT171850 NZ_CP078139:c4195171-4194914 [Pseudomonas savastanoi pv. savastanoi]
GTGGCAAACGTGAACGTAAGAAATCTACCCGATGAAGTGCATCGAGCAATCCGCATCCAAGCTGCCCTGCACGGGCGCAG
CACCGAGGCCGAAATACGCGACATCTTGGAGCGGGCGGCGAGACCCGAGGGACGTGTGAAACTTGGCTCGTTCCTAGCTT
CTATTGCCCGTGAGGTTGGTGGTCTGACCGATAAAGAACACACCTTGTTTGAGAACACGCGCATCACGTCTCCGGCCCGT
GCGGTGAGCTTTGAATGA

Structures

Toxin

• AlphaFold DB: U9ZUY2

Antitoxin

• AlphaFold DB: A0A5Y6U8W9