Detailed information of TA system TA171846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 54466..55091
• Replicon: plasmid pEC4-2
• Accession: NZ_CP060975
• Organism: Escherichia coli strain EC4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H9192_RS23865
• Protein ID: WP_000911313.1
• Coordinates: 54693..55091 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: H9192_RS23860
• Protein ID: WP_000450520.1
• Coordinates: 54466..54693 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9192_RS23860 (54466)	54466..54693	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
H9192_RS23865 (54693)	54693..55091	+	399	WP_000911313.1	type II toxin-antitoxin system VapC family toxin	Toxin
H9192_RS23870 (55100)	55100..57253	-	2154	WP_241461980.1	type IV conjugative transfer system coupling protein TraD	-
H9192_RS23875 (57506)	57506..58237	-	732	WP_023565183.1	conjugal transfer complement resistance protein TraT	-
H9192_RS23880 (58269)	58269..58766	-	498	WP_000605857.1	entry exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-14 / qacE / cmlA1 / mph(A) / oqxB / oqxA / sitABCD	iutA / iucD / iucC / iucB / iucA	1..112054	112054

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T171846 WP_000911313.1 NZ_CP060975:54693-55091 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T171846 NZ_CP078139:c139525-139250 [Pseudomonas savastanoi pv. savastanoi]
ATGGAATTGAAGTGGACAAGCAAGGCGCTTTCCGACATTACCAGGCTATACGAGTTTCTGGCCTTGGCGAATCAACCTGC
CGCAGCACGAGTAGTGCAGCAACTCACCACAGCACCTACCACGCTTCTGACTAACCCGCGCATTGGCGAACGCCTAGAGG
AGTTCGAGCCAAAGGACGTACGCCGAATTCAGATCGGCCAATACGAGATGCGTTACGAGATAGCGGATTCAACTATTTAC
CTGCTGCGCCTGTGGCACACCCGCGAAGACCGATAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT171846 WP_000450520.1 NZ_CP060975:54466-54693 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT171846 NZ_CP078139:c139797-139525 [Pseudomonas savastanoi pv. savastanoi]
ATGGCAACCACCGCCAAAACCCGATCTGTCACAGCTCACGTTCCGGTTCAGCTGGCGGAAAAAGTCGATTTGATGGCTGA
GCGTCTGGAGCGCTCTAAAAACTGGATCGTCAAGCAAGCCCTGTCTGCTTGGATAGACCAGGAAGAAGAGCGTAGTCGCC
TGACCCGTGAGGCCTTGGCTGATGTTGACGCTGGACGCGTTATTGATCACCAAGCCGTCCAGGCCTGGGCTGACAGTCTT
AGCACCGACACTCCGTTGCCGGTGCCGCGCTGA

Structures

Antitoxin

• AlphaFold DB: V0VCB2