Detailed information of TA system TA171841

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 7730..8289
• Replicon: plasmid pEC4-2
• Accession: NZ_CP060975
• Organism: Escherichia coli strain EC4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: H9192_RS23575
• Protein ID: WP_000421257.1
• Coordinates: 7730..8005 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: H9192_RS23580
• Protein ID: WP_001178089.1
• Coordinates: 8005..8289 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9192_RS23560 (3657)	3657..4766	-	1110	WP_000771475.1	SDR family oxidoreductase	-
H9192_RS23565 (5199)	5199..6152	-	954	WP_000280980.1	omptin family outer membrane protease	-
H9192_RS23570 (6983)	6983..7680	+	698	WP_095033700.1	IS1-like element IS1B family transposase	-
H9192_RS23575 (7730)	7730..8005	-	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H9192_RS23580 (8005)	8005..8289	-	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
H9192_RS23585 (8800)	8800..9504	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
H9192_RS23590 (9570)	9570..9857	+	288	Protein_10	transposase	-
H9192_RS23595 (10107)	10107..10982	+	876	WP_001617865.1	extended-spectrum class A beta-lactamase CTX-M-14	-
H9192_RS23600 (11017)	11017..11985	+	969	WP_013362812.1	IS5-like element IS903B family transposase	-
H9192_RS23605 (12042)	12042..12614	-	573	Protein_13	IS1380-like element ISEcp1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-14 / qacE / cmlA1 / mph(A) / oqxB / oqxA / sitABCD	iutA / iucD / iucC / iucB / iucA	1..112054	112054
	-	inside	IScluster/Tn	blaCTX-M-14 / qacE / cmlA1 / mph(A)	-	7303..23613	16310

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T171841 WP_000421257.1 NZ_CP060975:c8005-7730 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T171841 NZ_CP078137:2936196-2936738 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT171841 WP_001178089.1 NZ_CP060975:c8289-8005 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT171841 NZ_CP078137:2935901-2936212 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: S1PMX5

Antitoxin

• AlphaFold DB: A0A731IHA8