Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4185339..4186174 Replicon chromosome
Accession NZ_CP060974
Organism Escherichia coli strain EC4

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0J2AEA6
Locus tag H9192_RS20345 Protein ID WP_000854759.1
Coordinates 4185339..4185716 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1NM52
Locus tag H9192_RS20350 Protein ID WP_001295723.1
Coordinates 4185806..4186174 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9192_RS20315 (4180967) 4180967..4181713 - 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
H9192_RS20320 (4181728) 4181728..4183269 - 1542 WP_001298859.1 IS21-like element ISEc12 family transposase -
H9192_RS20325 (4183863) 4183863..4184039 - 177 Protein_3996 helix-turn-helix domain-containing protein -
H9192_RS20330 (4184406) 4184406..4184555 - 150 Protein_3997 hypothetical protein -
H9192_RS20335 (4184661) 4184661..4184837 - 177 WP_000839286.1 DUF957 domain-containing protein -
H9192_RS20340 (4184854) 4184854..4185342 - 489 WP_000761690.1 DUF5983 family protein -
H9192_RS20345 (4185339) 4185339..4185716 - 378 WP_000854759.1 TA system toxin CbtA family protein Toxin
H9192_RS20350 (4185806) 4185806..4186174 - 369 WP_001295723.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
H9192_RS20355 (4186337) 4186337..4186558 - 222 WP_000692345.1 DUF987 domain-containing protein -
H9192_RS20360 (4186621) 4186621..4187097 - 477 WP_001186775.1 RadC family protein -
H9192_RS20365 (4187113) 4187113..4187586 - 474 WP_001350782.1 antirestriction protein -
H9192_RS20370 (4187928) 4187928..4188746 - 819 WP_001234738.1 DUF932 domain-containing protein -
H9192_RS20375 (4188864) 4188864..4189059 - 196 Protein_4006 DUF905 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14144.26 Da        Isoelectric Point: 7.3249

>T171839 WP_000854759.1 NZ_CP060974:c4185716-4185339 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Download         Length: 378 bp

>T171839 NZ_CP078137:2056390-2056497 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13592.40 Da        Isoelectric Point: 6.6255

>AT171839 WP_001295723.1 NZ_CP060974:c4186174-4185806 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT171839 NZ_CP078137:c2056340-2056277 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2AEA6

Antitoxin

Source ID Structure
AlphaFold DB S1NM52

References