Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4114510..4115086 Replicon chromosome
Accession NZ_CP060974
Organism Escherichia coli strain EC4

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag H9192_RS19990 Protein ID WP_001297643.1
Coordinates 4114799..4115086 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag H9192_RS19985 Protein ID WP_000063156.1
Coordinates 4114510..4114812 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9192_RS19970 (4111106) 4111106..4113256 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
H9192_RS19975 (4113306) 4113306..4113509 + 204 WP_000467859.1 YbdD/YjiX family protein -
H9192_RS19980 (4113520) 4113520..4114476 + 957 WP_001297640.1 GTPase -
H9192_RS19985 (4114510) 4114510..4114812 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
H9192_RS19990 (4114799) 4114799..4115086 - 288 WP_001297643.1 BrnT family toxin Toxin
H9192_RS19995 (4115285) 4115285..4115982 - 698 Protein_3929 IS1 family transposase -
H9192_RS20000 (4116039) 4116039..4116134 + 96 Protein_3930 dienelactone hydrolase family protein -
H9192_RS20005 (4116280) 4116280..4117512 + 1233 WP_063104094.1 multidrug efflux MFS transporter MdtM -
H9192_RS20010 (4117553) 4117553..4118833 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T171838 WP_001297643.1 NZ_CP060974:c4115086-4114799 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T171838 NZ_CP078137:2055855-2055962 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT171838 WP_000063156.1 NZ_CP060974:c4114812-4114510 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT171838 NZ_CP078137:c2055808-2055743 [Escherichia coli]
TGTTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References