Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3516201..3516819 Replicon chromosome
Accession NZ_CP060974
Organism Escherichia coli strain EC4

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H9192_RS17065 Protein ID WP_001291435.1
Coordinates 3516601..3516819 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H9192_RS17060 Protein ID WP_000344800.1
Coordinates 3516201..3516575 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9192_RS17050 (3511290) 3511290..3512483 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H9192_RS17055 (3512506) 3512506..3515655 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
H9192_RS17060 (3516201) 3516201..3516575 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H9192_RS17065 (3516601) 3516601..3516819 + 219 WP_001291435.1 HHA domain-containing protein Toxin
H9192_RS17070 (3516991) 3516991..3517542 + 552 WP_000102564.1 maltose O-acetyltransferase -
H9192_RS17075 (3517658) 3517658..3518128 + 471 WP_000136192.1 YlaC family protein -
H9192_RS17080 (3518292) 3518292..3519842 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H9192_RS17085 (3519884) 3519884..3520237 - 354 WP_000878140.1 DUF1428 domain-containing protein -
H9192_RS17095 (3520616) 3520616..3520927 + 312 WP_000409911.1 MGMT family protein -
H9192_RS17100 (3520958) 3520958..3521530 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T171835 WP_001291435.1 NZ_CP060974:3516601-3516819 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T171835 NZ_CP078137:1438751-1438854 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT171835 WP_000344800.1 NZ_CP060974:3516201-3516575 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT171835 NZ_CP078137:c1438892-1438621 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References