Detailed information of TA system TA171834

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2525173..2525811
• Replicon: chromosome
• Accession: NZ_CP060974
• Organism: Escherichia coli strain EC4

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: H9192_RS12330
• Protein ID: WP_001447010.1
• Coordinates: 2525635..2525811 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: H9192_RS12325
• Protein ID: WP_001270286.1
• Coordinates: 2525173..2525589 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9192_RS12305 (2520324)	2520324..2521265	-	942	WP_001251304.1	ABC transporter permease	-
H9192_RS12310 (2521266)	2521266..2522279	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
H9192_RS12315 (2522297)	2522297..2523442	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
H9192_RS12320 (2523687)	2523687..2525094	-	1408	Protein_2421	PLP-dependent aminotransferase family protein	-
H9192_RS12325 (2525173)	2525173..2525589	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
H9192_RS12330 (2525635)	2525635..2525811	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
H9192_RS12335 (2526033)	2526033..2526263	+	231	WP_000494244.1	YncJ family protein	-
H9192_RS12340 (2526355)	2526355..2528316	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
H9192_RS12345 (2528389)	2528389..2528925	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
H9192_RS12350 (2529017)	2529017..2530192	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T171834 WP_001447010.1 NZ_CP060974:c2525811-2525635 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T171834 NZ_CP078137:1286231-1286485 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT171834 WP_001270286.1 NZ_CP060974:c2525589-2525173 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT171834 NZ_CP078137:1285983-1286234 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8