Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 21460..22103 Replicon plasmid pEC5-3
Accession NZ_CP060971
Organism Escherichia coli strain EC5

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag H9193_RS24210 Protein ID WP_001044768.1
Coordinates 21460..21876 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag H9193_RS24215 Protein ID WP_001261287.1
Coordinates 21873..22103 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9193_RS24195 (17959) 17959..18549 - 591 WP_000194575.1 hypothetical protein -
H9193_RS24200 (18549) 18549..18806 - 258 WP_000343085.1 hypothetical protein -
H9193_RS24205 (19160) 19160..21298 + 2139 WP_000350638.1 AAA family ATPase -
H9193_RS24210 (21460) 21460..21876 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
H9193_RS24215 (21873) 21873..22103 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
H9193_RS24220 (22399) 22399..22689 + 291 WP_000111771.1 hypothetical protein -
H9193_RS24225 (22679) 22679..23578 + 900 WP_000963206.1 nucleotide-binding protein -
H9193_RS24230 (23628) 23628..25853 - 2226 WP_000698737.1 P-loop NTPase fold protein -
H9193_RS24235 (25855) 25855..26943 - 1089 WP_000952217.1 transcriptional repressor PifC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR / fosA3 / blaTEM-1B / blaCTX-M-55 - 1..111472 111472


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T171811 WP_001044768.1 NZ_CP060971:c21876-21460 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T171811 NZ_CP078122:4251414-4251632 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT171811 WP_001261287.1 NZ_CP060971:c22103-21873 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT171811 NZ_CP078122:4251013-4251387 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References