Detailed information of TA system TA171803

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 91254..91879
• Replicon: plasmid pEC5-2
• Accession: NZ_CP060970
• Organism: Escherichia coli strain EC5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H9193_RS23735
• Protein ID: WP_000911313.1
• Coordinates: 91254..91652 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: H9193_RS23740
• Protein ID: WP_000450520.1
• Coordinates: 91652..91879 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9193_RS23720 (87579)	87579..88076	+	498	WP_000605857.1	entry exclusion protein	-
H9193_RS23725 (88108)	88108..88839	+	732	WP_023565183.1	conjugal transfer complement resistance protein TraT	-
H9193_RS23730 (89092)	89092..91245	+	2154	WP_000009324.1	type IV conjugative transfer system coupling protein TraD	-
H9193_RS23735 (91254)	91254..91652	-	399	WP_000911313.1	type II toxin-antitoxin system VapC family toxin	Toxin
H9193_RS23740 (91652)	91652..91879	-	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-14 / fosA3 / aac(3)-IVa / sul2 / floR / dfrA12 / aadA2 / qacE / mph(A) / aph(3')-Ia / blaTEM-1B / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..147909	147909

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T171803 WP_000911313.1 NZ_CP060970:c91652-91254 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T171803 NZ_CP078120:c3541511-3540204 [Halomonas sp. 18071143]
GTGGCTAACCTGACGGTAGCACTCAACGGTATCGAGGTAGGCCGACTCAGCCTCGGCCGTTCCGGCGCCATGGCTTTCCG
CTATCTGCCTGAATGGCTAAACCGCCCCGGCGCACGCGCTATTTCATTATCCATGCCGTTATCTTCGGCAAGCTATCGTG
GCGCGGTGGTTTACAACTTCTTCGATAACCTGCTGCCCGACTCCGAGGCGATTCGCGCCAGAATGCAGGCAAAATTTCAG
CTGGCGACCGGACACCCCTTTGACCTTCTCGCCGCCGTAGGTCGTGATTGTATCGGCGCTATTCAGCTGTATCCCGAGAC
AGACACCATACCCAATGTTCGACAGATCACAGCAGAGCCGCTGGATGAGACGGATATCGCGACATTGCTCAGCGGCTACC
AAGAGTTGCCGCTTGGCATGCATCGTGATGGAGACTTCAGAATCTCGCTCGCCGGGGCGCAGGAAAAAATGGCTCTGCTT
TGGCACAGGGAATGCTGGCATCGGCCATCATTTGCCACGCCCACCAGCCACATCTTCAAGCTGCCCATTGGGTTTCTCAG
TCATAGCAATATCGACCTTCGCGATAGCAACGAGAACGAATGGCTATGCCTGAAGATTCTCGAGGCCTTTGGAGTGCCGG
TTGCTAACGCCGAGCTGGCGCGCTTCGAACAGCATACCGTGCTGGTGGTAGCGCGATTCGACCGACGCTGGTCGAGCGAC
GGCAGCTGGTTGATGCGTCTCCCGCAGGAAGACTTTTGCCAGGCACTGGGCATTGCACCGGCACTGAAGTATGAGAACGA
CGGTGGGCCAGGTATCCGGGCAGGCATGGAATTGCTGATGGGCTCCCAGCAAGCCACGAGCGACCGGGAGAACTTCTTCA
AGGCTCAGATCATGTTCTGGCTGTTGGCCGCCATCGATGGGCATGCCAAGAATTTCAGTGTGTTCATCGAGCCACAAACT
GCTTACCGAATGACGCCGCTCTATGACGTTATTTCCGCCTATCCCCTCATGGCAAAAGGAAGCCTGCCCGAAGCAAAGGC
CAGGATGGCGATGTCGCTGAAAGGAAAGAACCGCCACTATCACTGGTCGCGTTTCTTGCCACGCCACTTTCTCAGTACGG
CCCAGCAGGTGGGGTTTTCTCGGCGGCGGACGCTGGAAATCATACAGAGCATACTCGCACAGGCTGACGCAGCCATGAGC
CGGGTAGCGGCGCTGCTGCCGGATGATTTTCCTGACACCGTGAGTGCCTCCATCATGGAAGGACTGGGTCGACAGCTCAA
GAGACTGGAAAAGGATATAGACCAATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT171803 WP_000450520.1 NZ_CP060970:c91879-91652 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT171803 NZ_CP078120:c3541763-3541515 [Halomonas sp. 18071143]
ATGAAAATTACCTCACCCGATATGCTGGCCAAGGCCTTGAAAGAAGCCCGCTCCCATAATGGCATGAGCCAGAAAGATGC
CGCCGAACAGGTCGGTATCAAACAAGCCACCGTCTCCGCTTTCGAGAATCGTCCGGAAAAAAGCCGCGTTGAAACGCTCT
TCAAACTGATGGCTGCCCTCGGACTAGAGTTACATGTGGCAAAGAGAGAGAGTAAGCAAAGTGAACAGGTGTGGGATGAG
GAGTGGTAA

Structures

Antitoxin

• AlphaFold DB: V0VCB2