Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4340778..4341275 Replicon chromosome
Accession NZ_CP060969
Organism Escherichia coli strain EC5

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag H9193_RS21125 Protein ID WP_001173343.1
Coordinates 4340778..4340951 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag H9193_RS21130 Protein ID WP_000398619.1
Coordinates 4340979..4341275 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9193_RS21115 (4337443) 4337443..4338900 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
H9193_RS21120 (4339137) 4339137..4340654 + 1518 WP_001295074.1 lysine--tRNA ligase -
H9193_RS21125 (4340778) 4340778..4340951 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
H9193_RS21130 (4340979) 4340979..4341275 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
H9193_RS21135 (4341502) 4341502..4341774 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
H9193_RS21140 (4341786) 4341786..4342016 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
H9193_RS21145 (4342197) 4342197..4343828 + 1632 WP_001216477.1 sensor histidine kinase -
H9193_RS21150 (4343825) 4343825..4344544 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T171802 WP_001173343.1 NZ_CP060969:c4340951-4340778 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T171802 NZ_CP078120:c3425161-3424979 [Halomonas sp. 18071143]
GTGAAAAGCAAGGATGTCATCAAGGAGATGGAAGCTGATGGATGGTATCTAGATCGTGTTAGTGGGAGCCATCACGTATT
CAAGCACCCTACCAAGCTCGGCAGTGTGCCCGTGCCTCATCCGAAAAAGGATTTGCCTAAGGGCACCATCCACAATATCA
GAAAACTTGCGGGGCTCAGGTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT171802 WP_000398619.1 NZ_CP060969:c4341275-4340979 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT171802 NZ_CP078120:c3424955-3424551 [Halomonas sp. 18071143]
ATGCAATATCCGGTTGCTATCGAATGGGGCGATGAACACACCGCCACCGGTATCGTCATTCCGGACATTCCCGGTGCTGT
GACAGCGGGTGACACGACCGAACATGCTTATGAAATGGCAGTGGAAGTGGCTCATATCCAGTTGGAAGAATTAGCCAACG
AGAATAAAGATATTCCCATGCCGCGCAGCATTGCGGAGCATCGCCAAAATCCAGAATTTGAAGGGTGCGGCTGGGGGATT
ATTGATATTGATATCACGCCCTACCTCGGCAAAACCGAAAAGGTGAATGTGACGCTGCCAGGTCACGTCATTCGTCAGAT
CGACCAATACGTGACCACCCACGGTATTAAAAGTCGTTCTGCGTTCATTGCCTCGGCGGCATTGGAAAAGCTGAACCACG
CGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References