Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3554765..3555383 Replicon chromosome
Accession NZ_CP060969
Organism Escherichia coli strain EC5

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H9193_RS17295 Protein ID WP_001291435.1
Coordinates 3555165..3555383 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H9193_RS17290 Protein ID WP_000344800.1
Coordinates 3554765..3555139 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9193_RS17280 (3549854) 3549854..3551047 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H9193_RS17285 (3551070) 3551070..3554219 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
H9193_RS17290 (3554765) 3554765..3555139 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H9193_RS17295 (3555165) 3555165..3555383 + 219 WP_001291435.1 HHA domain-containing protein Toxin
H9193_RS17300 (3555555) 3555555..3556106 + 552 WP_000102564.1 maltose O-acetyltransferase -
H9193_RS17305 (3556222) 3556222..3556692 + 471 WP_000136192.1 YlaC family protein -
H9193_RS17310 (3556856) 3556856..3558406 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H9193_RS17315 (3558448) 3558448..3558801 - 354 WP_000878140.1 DUF1428 domain-containing protein -
H9193_RS17325 (3559180) 3559180..3559491 + 312 WP_000409911.1 MGMT family protein -
H9193_RS17330 (3559522) 3559522..3560094 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T171797 WP_001291435.1 NZ_CP060969:3555165-3555383 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T171797 NZ_CP078120:2708479-2708661 [Halomonas sp. 18071143]
GTGAACAGCAGAGTACTGATCAAGGAACTGGAAGCGGATGGATGGACATTACTCAGAGTCAAAGGCAGCCACCACCACTT
CAGGCACCCGACCAAACCCGGCACCATCACCGTGCCACATCCGAAAAAGGATCTGAAAACGGGATTGGTCAAAGGAATCA
GGAAAAGCGCTGGCCTCGAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT171797 WP_000344800.1 NZ_CP060969:3554765-3555139 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT171797 NZ_CP078120:2708699-2709118 [Halomonas sp. 18071143]
ATGTTATTTCCCATTGCCATTGAACGCGGCGACGAACAACACGCCTATGGCGTCATCGTGCCCGACCTGCCCGGCTGCCA
CTCCGCAGGCGACACCTTCGAAGAGGCCCTGGCCAACGCCAAAGAAGCCATCGATGGCTGGCTGGAAGTGGCCGTTGAAT
ACGGCGATCCCATCCCCGAAGCCACGTCCATCGAACACCACATGGACAATCCCGACTTCACTGGCTGGATCTGGGCCGTC
GTCGATATAGACGTCACCCCGTACCTGGGCAAAAGCCACAAGATCAACGTCACCCTGCCCGACCTCCTGGTCAAACAGAT
CGACGACTTCGTCGCCCGCCACCCCGGCGACAAAACCCGCTCTGGCTTTCTCTCCCGCGTCGCCATGGCCGAACTGGCCA
GGAACAAACAAGCCAGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References