Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2457999..2458637 Replicon chromosome
Accession NZ_CP060969
Organism Escherichia coli strain EC5

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag H9193_RS11905 Protein ID WP_000813794.1
Coordinates 2458461..2458637 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag H9193_RS11900 Protein ID WP_001270286.1
Coordinates 2457999..2458415 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9193_RS11880 (2453151) 2453151..2454092 - 942 WP_001251313.1 ABC transporter permease -
H9193_RS11885 (2454093) 2454093..2455106 - 1014 WP_000220399.1 ABC transporter ATP-binding protein -
H9193_RS11890 (2455124) 2455124..2456269 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
H9193_RS11895 (2456514) 2456514..2457920 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
H9193_RS11900 (2457999) 2457999..2458415 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
H9193_RS11905 (2458461) 2458461..2458637 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
H9193_RS11910 (2458859) 2458859..2459089 + 231 WP_000494244.1 YncJ family protein -
H9193_RS11915 (2459181) 2459181..2461142 - 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
H9193_RS11920 (2461215) 2461215..2461751 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
H9193_RS11925 (2461843) 2461843..2463018 + 1176 WP_001236265.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T171795 WP_000813794.1 NZ_CP060969:c2458637-2458461 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T171795 NZ_CP078120:c2536032-2535730 [Halomonas sp. 18071143]
ATGAAAATAATTAGCGAAACCGACACTTATTCGGACTGGTTCGATAAATTACGCGATGCTCGGGCCAGAGCGAAAATTCT
GGCTCGTCTGCGCCGGGCTGAGCTGGGCAACTTCGGGGATTGTGAGCCGGTGGGGGAAGGAGTCTCGGAATTGAGGATTC
ACTACGGGCCTGGTTATCGAGTCTATTTTGTCCAGCGGGGGTATGAGCTGATTATTTTGTTGGCGGGCGGTGATAAATCC
AGCCAGGCAAAAGATATTGATATCGCGAAGCAATTGGCACGCGATTTGAAGGAGGCACCATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT171795 WP_001270286.1 NZ_CP060969:c2458415-2457999 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT171795 NZ_CP078120:c2535733-2535452 [Halomonas sp. 18071143]
ATGAGCACGCTGCGTAAATGGGATGTCGTTGACCACTTGAATACCGAAGAGGAAATGGTGTTGTACTTTGAAGCCTGCCT
TGAAGAGGGCGACGCTGCCCTGGTGGCGGCAGCGCTTGGCGATATTGCCCGCGCCCGTGGTATGGCACAGCTTGCTCGTG
ATACGGGGTTGACTCGCGAAGGGTTGTACAAGGCGCTTTCTGCTGATGGAAACCCAAGTTTTGCAACGATTATGAAGGTG
ACAAAAGCCTTGGGCTTTACGTTACGTGCTGAAGTATCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References