Detailed information of TA system TA171785

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1867582..1868417
• Replicon: chromosome
• Accession: NZ_CP060969
• Organism: Escherichia coli strain EC5

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: H9193_RS08855
• Protein ID: WP_064221650.1
• Coordinates: 1867582..1867959 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: H9193_RS08860
• Protein ID: WP_001295723.1
• Coordinates: 1868049..1868417 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9193_RS08820 (1863102)	1863102..1863575	+	474	WP_001105407.1	DNA gyrase inhibitor SbmC	-
H9193_RS08825 (1863773)	1863773..1864831	+	1059	WP_001200895.1	FUSC family protein	-
H9193_RS08830 (1865003)	1865003..1865332	+	330	WP_000450409.1	DUF496 family protein	-
H9193_RS08835 (1865433)	1865433..1865798	-	366	WP_001280454.1	EutP/PduV family microcompartment system protein	-
H9193_RS08840 (1866069)	1866069..1866458	-	390	WP_077875581.1	transposase	-
H9193_RS08845 (1867256)	1867256..1867336	-	81	Protein_1735	hypothetical protein	-
H9193_RS08850 (1867436)	1867436..1867585	-	150	Protein_1736	DUF5983 family protein	-
H9193_RS08855 (1867582)	1867582..1867959	-	378	WP_064221650.1	TA system toxin CbtA family protein	Toxin
H9193_RS08860 (1868049)	1868049..1868417	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
H9193_RS08865 (1868580)	1868580..1868801	-	222	WP_000692345.1	DUF987 domain-containing protein	-
H9193_RS08870 (1868864)	1868864..1869340	-	477	WP_001186775.1	RadC family protein	-
H9193_RS08875 (1869356)	1869356..1869841	-	486	WP_000849588.1	antirestriction protein	-
H9193_RS08880 (1869896)	1869896..1870714	-	819	WP_072649824.1	DUF932 domain-containing protein	-
H9193_RS08885 (1870832)	1870832..1871027	-	196	Protein_1743	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14150.14 Da
• Isoelectric Point: 7.3249

>T171785 WP_064221650.1 NZ_CP060969:c1867959-1867582 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTQLARLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T171785 NZ_CP078118:c2153724-2153542 [Neisseria gonorrhoeae]
TTGAATAGCCTAGACGTTATTGCCCTACTCAAACAAGACGGTTGGTATAAAGTTGCACAATCCGGGAGCCATTCGCAATA
TAAGCACCCAACAAAAAAAGGCCGTGTAACCGTGCCGCACCCGAAAAAAGACTTGCCGACAGGTACTGTAAAAAATATCT
ATAAGCAAGCCGGTTTGAAGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT171785 WP_001295723.1 NZ_CP060969:c1868417-1868049 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT171785 NZ_CP078118:c2153440-2153039 [Neisseria gonorrhoeae]
ATGTTTATCCCTGCCGCCCTGCACAAAGACGAACATTCGGCATATGGCGTAACCATCCCCGACCTTCCCGGCTGTTTCTC
TTGCGGCGACACCGTTGAAGAAGCCGTCGCCAACGCCCGTTCTGCCGCCTATATGCATATTGACGGTATGATTGAGGACG
GAGGGTTCAAAAACCTTGCCGTAAGCAGCATTGCCGATTTGAGCCAAGAACCTGATTATCATGGGGCAACATGGGTAATG
ATTGAAATCGACCCTGCAAAAATCAGTCGGCAGCAAATCCGATTCAACGTCAGCTGGCCGCAATACCTTCTTGATAGAGT
GGATGAATATACATCCGCAAATCATGAAACCCGTAGCGGTTTTTTAGCCAAAGCCGCCCTGCTCACCATGAATCAGGCAT
AG

Structures

Antitoxin

• AlphaFold DB: S1NM52