Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 750667..751508 Replicon chromosome
Accession NZ_CP060969
Organism Escherichia coli strain EC5

Toxin (Protein)


Gene name yeeV Uniprot ID A0A1M0WJM1
Locus tag H9193_RS03640 Protein ID WP_000854822.1
Coordinates 751125..751508 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A1M0CWL2
Locus tag H9193_RS03635 Protein ID WP_053271974.1
Coordinates 750667..751035 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9193_RS03615 (748412) 748412..749230 + 819 WP_001234702.1 DUF932 domain-containing protein -
H9193_RS03620 (749321) 749321..749806 + 486 WP_000214317.1 antirestriction protein -
H9193_RS03625 (749821) 749821..750297 + 477 WP_001186747.1 RadC family protein -
H9193_RS03630 (750366) 750366..750587 + 222 WP_000692312.1 DUF987 domain-containing protein -
H9193_RS03635 (750667) 750667..751035 + 369 WP_053271974.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
H9193_RS03640 (751125) 751125..751508 + 384 WP_000854822.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
H9193_RS03645 (751499) 751499..751987 + 489 WP_001177592.1 DUF5983 family protein -
H9193_RS03650 (751999) 751999..752196 + 198 WP_000839263.1 DUF957 domain-containing protein -
H9193_RS03655 (752293) 752293..753138 + 846 WP_040234917.1 DUF4942 domain-containing protein -
H9193_RS03665 (753437) 753437..753943 + 507 WP_000228937.1 G/U mismatch-specific DNA glycosylase -
H9193_RS03670 (754022) 754022..755863 - 1842 WP_000437375.1 RNA polymerase sigma factor RpoD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14295.32 Da        Isoelectric Point: 8.2830

>T171780 WP_000854822.1 NZ_CP060969:751125-751508 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHQESKRCN

Download         Length: 384 bp

>T171780 NZ_CP060969:751125-751508 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCAGGAGTCGAAACGATGCAACTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13679.41 Da        Isoelectric Point: 6.4758

>AT171780 WP_053271974.1 NZ_CP060969:750667-751035 [Escherichia coli]
VSDTFSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLTDRAGIRGRFSDADANHLDQAFPLLMKQLELMLTSS
ELNPHRQNTVTLYVKGLTCHADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT171780 NZ_CP060969:750667-751035 [Escherichia coli]
GTGTCAGACACATTCTCCGGGACAACGCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGTACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTACAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCGGATGCGAACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCAGT
GAACTGAATCCCCACCGTCAGAATACCGTCACGCTGTATGTTAAAGGGCTGACCTGCCATGCGGACACCCTCGGCAGCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M0WJM1

Antitoxin

Source ID Structure
AlphaFold DB A0A1M0CWL2

References