Detailed information of TA system TA171777

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 13171..13636
• Replicon: plasmid pEC7-mcr-1
• Accession: NZ_CP060967
• Organism: Escherichia coli strain EC7

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9ZUY2
• Locus tag: H9194_RS25435
• Protein ID: WP_001230707.1
• Coordinates: 13385..13636 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: M7CCF8
• Locus tag: H9194_RS25430
• Protein ID: WP_000801440.1
• Coordinates: 13171..13398 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9194_RS25390 (H9194_25185)	8402..8617	-	216	WP_000760375.1	EexN family lipoprotein	-
H9194_RS25395 (H9194_25190)	8614..8907	-	294	WP_060598542.1	sigma factor-like helix-turn-helix DNA-binding protein	-
H9194_RS25400 (H9194_25195)	8984..10213	-	1230	WP_001329504.1	DNA relaxase/nickase TaxC	-
H9194_RS25405 (H9194_25200)	10217..10720	-	504	WP_000781813.1	relaxosome-coupling protein TaxA	-
H9194_RS25410 (H9194_25205)	11081..11428	-	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	-
H9194_RS25415 (H9194_25210)	11425..11672	-	248	Protein_13	type II toxin-antitoxin system HicA family toxin	-
H9194_RS25420 (H9194_25215)	11977..12327	-	351	WP_000854259.1	hypothetical protein	-
H9194_RS25425 (H9194_25220)	12418..12717	-	300	WP_000650309.1	hypothetical protein	-
H9194_RS25430 (H9194_25225)	13171..13398	-	228	WP_000801440.1	hypothetical protein	Antitoxin
H9194_RS25435 (H9194_25230)	13385..13636	-	252	WP_001230707.1	hypothetical protein	Toxin
H9194_RS25440 (H9194_25235)	13633..14175	-	543	WP_001083906.1	hypothetical protein	-
H9194_RS25445 (H9194_25240)	14280..14795	-	516	WP_001025395.1	J domain-containing protein	-
H9194_RS25450 (H9194_25250)	16294..17076	+	783	WP_000729201.1	replication initiation protein	-
H9194_RS25455 (H9194_25255)	17089..17493	+	405	WP_012421203.1	hypothetical protein	-
H9194_RS25460 (H9194_25260)	17628..18155	+	528	WP_000577043.1	DUF2726 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-1.1	-	1..33308	33308

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-126)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9751.13 Da
• Isoelectric Point: 8.9187

>T171777 WP_001230707.1 NZ_CP060967:c13636-13385 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Nucleotide

• Length: 252 bp

>T171777 NZ_CP078114:107000-107182 [Neisseria gonorrhoeae]
TTGAATAGCCTAGACGTTATTGCCCTACTCAAACAAGACGGTTGGTATAAAGTTGCACAATCCGGGAGCCATTCGCAATA
TAAGCACCCAACAAAAAAAGGCCGTGTAACCGTGCCGCACCCGAAAAAAGACTTGCCGACAGGTACTGTAAAAAATATCT
ATAAGCAAGCCGGTTTGAAGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8659.98 Da
• Isoelectric Point: 8.5105

>AT171777 WP_000801440.1 NZ_CP060967:c13398-13171 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Nucleotide

• Length: 228 bp

>AT171777 NZ_CP078114:107284-107685 [Neisseria gonorrhoeae]
ATGTTTATCCCTGCCGCCCTGCACAAAGACGAACATTCGGCATATGGCGTAACCATCCCCGACCTTCCCGGCTGTTTCTC
TTGCGGCGACACCGTTGAAGAAGCCGTCGCCAACGCCCGTTCTGCCGCCTATATGCATATTGACGGTATGATTGAGGACG
GAGGGTTCAAAAACCTTGCCGTAAGCAGCATTGCCGATTTGAGCCAAGAACCTGATTATCATGGGGCAACATGGGTAATG
ATTGAAATCGACCCTGCAAAAATCAGTCGGCAGCAAATCCGATTCAACGTCAGCTGGCCGCAATACCTTCTTGATAGAGT
GGATGAATATACATCCGCAAATCATGAAACCCGTAGCGGTTTTTTAGCCAAAGCCGCCCTGCTCACCATGAATCAGGCAT
AG

Structures

Toxin

• AlphaFold DB: U9ZUY2

Antitoxin

• AlphaFold DB: A0A5Y6U8W9