Detailed information of TA system TA171769

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 75602..76227
• Replicon: plasmid pEC7-2
• Accession: NZ_CP060964
• Organism: Escherichia coli strain EC7

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H9194_RS24230
• Protein ID: WP_000911313.1
• Coordinates: 75829..76227 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: H9194_RS24225
• Protein ID: WP_000450520.1
• Coordinates: 75602..75829 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9194_RS24225 (75602)	75602..75829	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
H9194_RS24230 (75829)	75829..76227	+	399	WP_000911313.1	type II toxin-antitoxin system VapC family toxin	Toxin
H9194_RS24235 (76236)	76236..77609	-	1374	Protein_75	type IV secretion system DNA-binding domain-containing protein	-
H9194_RS24240 (77653)	77653..78357	-	705	WP_001067858.1	IS6-like element IS26 family transposase	-
H9194_RS24245 (78677)	78677..79852	+	1176	WP_002914189.1	multidrug efflux RND transporter periplasmic adaptor subunit OqxA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-14 / fosA3 / aac(3)-IVa / sul2 / floR / oqxA / oqxB / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..128054	128054

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T171769 WP_000911313.1 NZ_CP060964:75829-76227 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T171769 NZ_CP078110:3704168-3704473 [Cronobacter sakazakii]
ATGAAGCATATTTTTCAAACTGAAGTATTCCGCCGTTGGCAAGCCAGTCTGAAAGACCAGCGGGTAAAAACGATGATTGC
CGCGCGGCTTTTTCGGCTGGCTAATGGTTTGCCTGGTGATGTTAAGCCCGTCGGCGCAGGCATCAGTGAACTGCGTATTC
ACTACGGCGCGGGTTACCGGATTTATTTCAAACAACACGGCGAGGTGATTGTCATTTTGCTTTGTGGTGGTGATAAGCAC
ACGCAGGATAAAGACATTCTTCTTGCGAAAATGCTGGCTGACCTGCCAGAGAACCAGTGGAGATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT171769 WP_000450520.1 NZ_CP060964:75602-75829 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT171769 NZ_CP078110:3704478-3704753 [Cronobacter sakazakii]
ATGAAGCTGACCGAATACGATCCCGCAGCGGCGCTGGTAGATGATGAGGAGATCGCCTTCTTTATGGCGGACGCGCTCGA
AACTGGCGACGCCGGACATATTGCAAAGGCGCTGGGCGTCGTCGCGCGCGCTAAAGGCATGTCGCATATCGCCGCTCAGA
CAGGGCTGTCGCGTGAACAGCTGTACCGTTCTTTCAGTGATAAAGGCAACCCAACGCTTAAAACCACGCTGGCAGTGATG
AAAGCGTTGGGTCTCGGTTTAACGGTTAAATCATAA

Structures

Antitoxin

• AlphaFold DB: V0VCB2