Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4374631..4375128 Replicon chromosome
Accession NZ_CP060963
Organism Escherichia coli strain EC7

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag H9194_RS21635 Protein ID WP_001173343.1
Coordinates 4374631..4374804 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag H9194_RS21640 Protein ID WP_000398619.1
Coordinates 4374832..4375128 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9194_RS21625 (4371301) 4371301..4372758 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
H9194_RS21630 (4372995) 4372995..4374512 + 1518 WP_001295074.1 lysine--tRNA ligase -
H9194_RS21635 (4374631) 4374631..4374804 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
H9194_RS21640 (4374832) 4374832..4375128 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
H9194_RS21645 (4375355) 4375355..4375627 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
H9194_RS21650 (4375639) 4375639..4375869 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
H9194_RS21655 (4376050) 4376050..4377681 + 1632 WP_001216477.1 sensor histidine kinase -
H9194_RS21660 (4377678) 4377678..4378397 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4345280..4375128 29848


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T171768 WP_001173343.1 NZ_CP060963:c4374804-4374631 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T171768 NZ_CP078110:c3703951-3703601 [Cronobacter sakazakii]
ATGATAAAACCGTTGTTTTGGGTCGGGCAGGCCAGAAAAGATCTTCAGGCGCTGCCGGAGGATGTACAGGATCTTTTTGG
TTATGCGCTTTATCTGGCGCAGCAGGGCAGGCGCCATCCGCAGGCTAAACCCCTTAAAGGATTCGGTGGCGCGGACATAC
TTGAGGTGGTGGAGGATTATCAGGGGAATGCCTTTCGCGCGGTCTATACCGTGTGCCTGAGAGATGCGATTTATATCCTG
CATGTATTCCAGAAAAAATCCTCTTCCGGGATTGCCACGCCCCGCCCGGAGATGGAAAAAATTGAACAGCGACTTAAAGC
CGCGCAACGCCACGCCGGAGGGCTGAAATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT171768 WP_000398619.1 NZ_CP060963:c4375128-4374832 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT171768 NZ_CP078110:c3703604-3703281 [Cronobacter sakazakii]
ATGACAGCAGACATTGAACGCAGTAACGGAAATGTGTATGACGATCTCGGGCTTATCGACGCCGAAGAGATGCAGCTAAA
AGCGCAGCTCGCTATGGCCATTAGCGACATTCTTAAAGCGCGCGGTTTAACCCAACAGGAGGCGGCCCGTCTTCTGGGAA
TGACCCAGCCAAAACTCTCTTTATTACTTCGCGGCCAGTTTCGCGGGATCAGCGAAACGAAAATGATGGCCTGCCTGATG
CTCCTCGGGCGGGATATCAGCATCGTCGTGGGCGAGGCACAGCAGGCGCGCGGAAAAATGCGCCTGATTTATGAGCCAGC
CTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References