Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4070786..4071362 Replicon chromosome
Accession NZ_CP060963
Organism Escherichia coli strain EC7

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag H9194_RS20100 Protein ID WP_001297643.1
Coordinates 4071075..4071362 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag H9194_RS20095 Protein ID WP_000063156.1
Coordinates 4070786..4071088 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9194_RS20080 (4067382) 4067382..4069532 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
H9194_RS20085 (4069582) 4069582..4069785 + 204 WP_000467859.1 YbdD/YjiX family protein -
H9194_RS20090 (4069796) 4069796..4070752 + 957 WP_001297640.1 GTPase -
H9194_RS20095 (4070786) 4070786..4071088 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
H9194_RS20100 (4071075) 4071075..4071362 - 288 WP_001297643.1 BrnT family toxin Toxin
H9194_RS20105 (4071561) 4071561..4072258 - 698 Protein_3950 IS1 family transposase -
H9194_RS20110 (4072315) 4072315..4072410 + 96 Protein_3951 dienelactone hydrolase family protein -
H9194_RS20115 (4072556) 4072556..4073788 + 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
H9194_RS20120 (4073829) 4073829..4075109 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T171766 WP_001297643.1 NZ_CP060963:c4071362-4071075 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T171766 NZ_CP078110:c2808408-2807923 [Cronobacter sakazakii]
GTGGGACGTGTAACAGCACCAGAACCATTATCCAGCTCCCATCAACTAGCAGAGTTCGTCAGTGGTGAGGCAGTACTTGA
TGACTGGCTTAAACAGAGGGGATTAAAAAACCAAGCTCTTGGTGCAGCACGAACGTTCGTCGTTTGTAAAAGAGACACTA
AACAGGTTGTCGGTTTTTACTCTCTGGCCACCGGCAGCGTTAACCACGTGGAAGCAATGGGAAGCCTCAGGCGCAATATG
CCAGATCCAATTCCCGTAATCATACTTGCTCGCCTGGCAGTTGATGTTTCATACCGTGGCCAAGGGCTCGGGGCCGATTT
ACTGCATGATGCAGTTCTGCGCTGTTACCGTGTAGCTGAGAATATTGGAGTCCGTGCGATTATGGTTCATGCACTGACTG
AAGAAGCCAAAAACTTTTATCTTCACCATGGCTTTAAGGCATCACAAACTCAGGAACGGACATTGTTTTTAAAGTTACCA
CAGTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT171766 WP_000063156.1 NZ_CP060963:c4071088-4070786 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT171766 NZ_CP078110:c2808662-2808396 [Cronobacter sakazakii]
ATGAAATCAGATGTTCAGCTCAACCTTAGAGCTAAAGAATCTCAAAGAGCACTTATTGATGCTGCAGCAGAGATTCTCCA
TAAATCACGCACGGACTTCATCCTGGAAATGGCATGTCAGGCTGCGGAGAATGTGATCCTCGATCGTCGCGTATTTAATT
TTAACGATGAGCAGTATGCAGAGTTTATCGATATGCTCGATGCACCGGTTTCGGATGAGGCGGCTATTGATAAACTTCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References