Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3517167..3517785 Replicon chromosome
Accession NZ_CP060963
Organism Escherichia coli strain EC7

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H9194_RS17355 Protein ID WP_001291435.1
Coordinates 3517567..3517785 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H9194_RS17350 Protein ID WP_000344800.1
Coordinates 3517167..3517541 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9194_RS17340 (3512256) 3512256..3513449 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H9194_RS17345 (3513472) 3513472..3516621 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
H9194_RS17350 (3517167) 3517167..3517541 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H9194_RS17355 (3517567) 3517567..3517785 + 219 WP_001291435.1 HHA domain-containing protein Toxin
H9194_RS17360 (3517957) 3517957..3518508 + 552 WP_000102564.1 maltose O-acetyltransferase -
H9194_RS17365 (3518624) 3518624..3519094 + 471 WP_000136192.1 YlaC family protein -
H9194_RS17370 (3519258) 3519258..3520808 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H9194_RS17375 (3520850) 3520850..3521203 - 354 WP_000878140.1 DUF1428 domain-containing protein -
H9194_RS17385 (3521582) 3521582..3521893 + 312 WP_000409911.1 MGMT family protein -
H9194_RS17390 (3521924) 3521924..3522496 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T171763 WP_001291435.1 NZ_CP060963:3517567-3517785 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T171763 NZ_CP078110:c2262657-2262554 [Cronobacter sakazakii]
GGCAAGGCGACTGAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTTTTTTT

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT171763 WP_000344800.1 NZ_CP060963:3517167-3517541 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT171763 NZ_CP078110:2262551-2262719 [Cronobacter sakazakii]
GATAAAAAAAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGG
CATTAATGCAGGCTCAGTCGCCTTGCCTTTTAAGAATAGATGACGACACCAGCTTTTCCAGTTTGCGGCTAAAGTCGCAG
CAAAAAGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References