Detailed information of TA system TA171762

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2448849..2449487
• Replicon: chromosome
• Accession: NZ_CP060963
• Organism: Escherichia coli strain EC7

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: H9194_RS12090
• Protein ID: WP_001447010.1
• Coordinates: 2449311..2449487 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: H9194_RS12085
• Protein ID: WP_001270286.1
• Coordinates: 2448849..2449265 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9194_RS12065 (2444001)	2444001..2444942	-	942	WP_001251304.1	ABC transporter permease	-
H9194_RS12070 (2444943)	2444943..2445956	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
H9194_RS12075 (2445974)	2445974..2447119	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
H9194_RS12080 (2447364)	2447364..2448770	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
H9194_RS12085 (2448849)	2448849..2449265	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
H9194_RS12090 (2449311)	2449311..2449487	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
H9194_RS12095 (2449709)	2449709..2449939	+	231	WP_000494244.1	YncJ family protein	-
H9194_RS12100 (2450031)	2450031..2451992	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
H9194_RS12105 (2452065)	2452065..2452601	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
H9194_RS12110 (2452693)	2452693..2453868	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T171762 WP_001447010.1 NZ_CP060963:c2449487-2449311 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T171762 NZ_CP078110:1864824-1865102 [Cronobacter sakazakii]
ATGATCAGAAGCTTCAGGCATAAAGGCCTGCAACGCTATTTCGAAACGGGTTCCACCGCAGGCATTCATGCCCCCCATGC
CAGAAAGATTTCTCTGCGGCTGGCGGTGCTGAATCAGGCGATAAGACCCGCTGACGTCGACTTACCGGGATTTTTCCTGC
ATCCCCTGACGGGGGATCGTAAAGGTATATGGGCTGTGACCGTCAGCGGAAACTGGCGCATCACGTTTGAGTTTCGGGAT
GGCGATGTCTTTATCGTCAACTATGAGGATTACCACTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT171762 WP_001270286.1 NZ_CP060963:c2449265-2448849 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT171762 NZ_CP078110:1865102-1865389 [Cronobacter sakazakii]
ATGGCAACGATGTTTAACCCACCGCACCCGGGCAGGCTGGTGCAGGAGTCAATGGAAGCGCTCGGCCTCAGCGCCCGTGC
GCTGGCCAAAGCGCTGGACGTCGCGCCGTCGACGGTGCAGCGTCTGCTGGTCGGCAAATCTGACGTCTCCCCGGAAATGG
CGCTGCGTCTCTCGGCAGTCCTTGGCAGCTCGCCGCATGTCTGGCTGGGTATGCAAAACGAATACGATCTCTTTCAGGCG
CGCCAGTCTGTTGATTTCTCGCACCTGCAACGGTTGCAGAGGGCATAA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8