Detailed information of TA system TA171749

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 958180..958763
• Replicon: chromosome
• Accession: NZ_CP060963
• Organism: Escherichia coli strain EC7

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: H9194_RS04645
• Protein ID: WP_049253269.1
• Coordinates: 958428..958763 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A1V2T5M9
• Locus tag: H9194_RS04640
• Protein ID: WP_000581941.1
• Coordinates: 958180..958428 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9194_RS04630 (954519)	954519..955820	+	1302	WP_023281448.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
H9194_RS04635 (955868)	955868..958102	+	2235	WP_000226815.1	GTP pyrophosphokinase	-
H9194_RS04640 (958180)	958180..958428	+	249	WP_000581941.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
H9194_RS04645 (958428)	958428..958763	+	336	WP_049253269.1	endoribonuclease MazF	Toxin
H9194_RS04650 (958834)	958834..959625	+	792	WP_001071648.1	nucleoside triphosphate pyrophosphohydrolase	-
H9194_RS04655 (959853)	959853..961490	+	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
H9194_RS04660 (961578)	961578..962876	+	1299	WP_000036723.1	phosphopyruvate hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12197.18 Da
• Isoelectric Point: 8.7218

>T171749 WP_049253269.1 NZ_CP060963:958428-958763 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQARHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T171749 NZ_CP078092:c2714014-2713613 [Geobacter sulfurreducens]
ATGAAATTGCTTCTCGACACCAATATCTGCATTTACATCATAAAGCAGCAGCCGGTTGCTGTACTTGAGCGTTTCTTAGA
GTACCAGGTTGGCGATATTGGCATTTCATCCATAACTCTCTCGGAATTGCGCTACGGAGTTGCGAAGAGCACCCATATGG
AGAAAAACGCCAAGGCCCTCGACGAATTCATCATTCCTTTGGAATTGGTTTCTTATGATGAATCGGCGGCTCATGTCTAT
GGAGATATTCGAGCAGCTCTTGAAAAAGCTGGTACGCCCATTGGCTCCATGGATATGTTGATTGCCGCTCACGCTGTATC
GTTAGGAATCCCTCTGGTCACTAATAACACCCGCGAGTTTTTACGGGTCCCTTCCCTCAATATCATTGATTGGACGGTTT
AG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9385.76 Da
• Isoelectric Point: 4.4665

>AT171749 WP_000581941.1 NZ_CP060963:958180-958428 [Escherichia coli]
MIHSSVKRWGNSPAVRIPTTLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT171749 NZ_CP078092:c2714241-2714014 [Geobacter sulfurreducens]
ATGAAAACTGCAAAGATTTTTCAAAATGGACAAAGTCAGGCAGTAAGGCTTCCCAAAGAATTTCGCTTCGAAGACAGCGA
AGTATTCATCAAGAAAAGCGGCAACGTCGTTCAACTGATCCCTCGGAGCGATTCGTGGAATTCCCTCTTCGGCAGTCTCA
AGAAATTCTCCCGCGATTTCATGTCCGAGCGCATACAACCTGAACTGGACAAGCGGGATGGCTTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1V2T5M9