Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 820058..820712 Replicon chromosome
Accession NZ_CP060963
Organism Escherichia coli strain EC7

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag H9194_RS04020 Protein ID WP_000244777.1
Coordinates 820305..820712 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag H9194_RS04015 Protein ID WP_000354046.1
Coordinates 820058..820324 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9194_RS03990 (815227) 815227..815970 + 744 WP_000951962.1 SDR family oxidoreductase -
H9194_RS03995 (816027) 816027..817460 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
H9194_RS04000 (817505) 817505..817816 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
H9194_RS04005 (817980) 817980..818639 + 660 WP_000250274.1 hemolysin III family protein -
H9194_RS04010 (818835) 818835..819815 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
H9194_RS04015 (820058) 820058..820324 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
H9194_RS04020 (820305) 820305..820712 + 408 WP_000244777.1 protein YgfX Toxin
H9194_RS04025 (820752) 820752..821273 - 522 WP_001055874.1 flavodoxin FldB -
H9194_RS04030 (821385) 821385..822281 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
H9194_RS04035 (822306) 822306..823016 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
H9194_RS04040 (823022) 823022..824755 + 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T171748 WP_000244777.1 NZ_CP060963:820305-820712 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T171748 NZ_CP078092:2676697-2676987 [Geobacter sulfurreducens]
ATGAGCTATAGCCTCGAATTCAAGGAATCCGCCCTGAAGGAGTGGAAAAAACTCGATGGCAGAATCCGTGAACAGTTTAA
GAAGAAGCTTACCGAACGGCTTGAGCTTCCTCGCGTCGAGCCGGCCCGACTCTCCGGCATGCCCGATTGCTACAAAATCA
AGCTGAAGAACGCCGGCTACCGGCTGGTTTACCAGGTTGACGACAACCGCATCATCGTTACTGTCGTGGCTGTCGGAAAA
CGCGAAAACCTTACCGTTTATCGAGCGGCAGAGAAAAGGGTGAAACAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT171748 WP_000354046.1 NZ_CP060963:820058-820324 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT171748 NZ_CP078092:2676462-2676707 [Geobacter sulfurreducens]
ATGGAACGTCTTTTTGCCCGTGCCTCGGTCAGCATAAGTGACCTTAAAAAAAACCCCACCCGAATCATTAATCAGTCCGA
AGGTGAACCTGTGGCAATCCTCAACCACAACAAGCCAAGTGCATACTTAATCCCCGCCGATGCCTTCGAGGCGCTCATGG
AGAAACTGGAGGATTATGAGCTTTCCCAAATTGTGAAGGAACGGGAACACGAGCCGAGTGTCAAGGTCTCCCTCGATGAG
CTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References