171744

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 86092..86617 Replicon plasmid pEC8-3
Accession NZ_CP060955
Organism Escherichia coli strain EC8

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag H9195_RS26235 Protein ID WP_001159868.1
Coordinates 86092..86397 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag H9195_RS26240 Protein ID WP_000813634.1
Coordinates 86399..86617 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9195_RS26220 (H9195_25965) 82002..83168 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
H9195_RS26225 (H9195_25970) 83756..84511 - 756 WP_000852146.1 replication initiation protein RepE -
H9195_RS26230 (H9195_25975) 85285..86091 - 807 WP_000016982.1 site-specific integrase -
H9195_RS26235 (H9195_25980) 86092..86397 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
H9195_RS26240 (H9195_25985) 86399..86617 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H9195_RS26245 (H9195_25990) 87325..88320 + 996 WP_000246636.1 hypothetical protein -
H9195_RS26250 (H9195_25995) 88363..89256 + 894 WP_001553853.1 S-4TM family putative pore-forming effector -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / aac(3)-IId / blaTEM-1B senB 1..97755 97755


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T171744 WP_001159868.1 NZ_CP060955:c86397-86092 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T171744 NZ_CP078081:2324952-2325689 [Bacillus tropicus]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGTAATCGTTTTTCTAGTACTTGTTTTCTTTTATTGGAAAAACGA
AGAGTTGTACGAGGAGAAGAAGCAACGTATTAGAAAGACTTGGTATGGGTTGTTTATAATATCAGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCGAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATACGTTCTCAATTTAGTACTATGATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT171744 WP_000813634.1 NZ_CP060955:c86617-86399 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT171744 NZ_CP078081:2325802-2325927 [Bacillus tropicus]
GTGATAAAAAATAATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACGGATTTCTCGTTAATTAAAGGGGATAC
GATGCAGCAAGTAGAAGATTTAGAAGATGAATTAAAAAGAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References