Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4141883..4142501 Replicon chromosome
Accession NZ_CP060953
Organism Escherichia coli strain EC8

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H9195_RS19995 Protein ID WP_001291435.1
Coordinates 4141883..4142101 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H9195_RS20000 Protein ID WP_000344800.1
Coordinates 4142127..4142501 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9195_RS19960 (4137171) 4137171..4137743 + 573 WP_000779831.1 YbaY family lipoprotein -
H9195_RS19965 (4137774) 4137774..4138085 - 312 WP_000409908.1 MGMT family protein -
H9195_RS19975 (4138464) 4138464..4138817 + 354 WP_000878140.1 DUF1428 domain-containing protein -
H9195_RS19980 (4138859) 4138859..4140409 - 1551 WP_001538392.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H9195_RS19985 (4140573) 4140573..4141043 - 471 WP_000136192.1 YlaC family protein -
H9195_RS19990 (4141159) 4141159..4141710 - 552 WP_000102539.1 maltose O-acetyltransferase -
H9195_RS19995 (4141883) 4141883..4142101 - 219 WP_001291435.1 HHA domain-containing protein Toxin
H9195_RS20000 (4142127) 4142127..4142501 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H9195_RS20005 (4143047) 4143047..4146196 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -
H9195_RS20010 (4146219) 4146219..4147412 - 1194 WP_001538394.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T171741 WP_001291435.1 NZ_CP060953:c4142101-4141883 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T171741 NZ_CP078071:c2834259-2833960 [Burkholderia sp. EMB26]
ATGGACAAGATCAAGACGCGGCCGTGGGATTCGGCCGAACACCTGAAAACCGAAGACGACATGGCCGACTACTTCGACGC
ATGCCTGCAGGAGGCCGGCGACGATCCGGCCTTCATCGCGCATGCGCTCGGCGTGATCGCCCGCGCACGCGGCATGTCGC
AGGTCGCACGCGATGCGGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACG
ATCCTGAAAGTCATCAAGGCGCTCGGCCTGCAATTGCACGGCGCCAAGGCTGCCGCGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT171741 WP_000344800.1 NZ_CP060953:c4142501-4142127 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT171741 NZ_CP078071:c2834601-2834263 [Burkholderia sp. EMB26]
ATGGTTGACACATTGCAATGCATGCCCTACAGTCCTCCTTCATTCGGCATCCGGACCATCGACGTCTTCGACGCCTGGTT
TGCCGGCCTGCCCGATCGCCTCGCCAAACGCCGCATCCAGGCGCGCATCGACCGCCTGTCGATGGGCAATCCGGGCAACT
GGAAATCCGCCGGCTCCCCCGTCGTCGAAATGCGTATCGACCACGGCCCCGGCTATCGCGTCTATGACGTGAGGCGCGGA
ACGATATGGGTCATCCTGCTGTGCGGCGGCGATCAATCGACGCAACAGGCCGACATCCGCGCCGCGCACGCAATGCTCGC
GCACCTCGACATGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References