Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3667245..3667795 Replicon chromosome
Accession NZ_CP060953
Organism Escherichia coli strain EC8

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag H9195_RS17745 Protein ID WP_001160966.1
Coordinates 3667481..3667795 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag H9195_RS17740 Protein ID WP_000125568.1
Coordinates 3667245..3667478 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9195_RS17715 (3662318) 3662318..3662605 + 288 WP_000203741.1 ferredoxin-like protein FixX -
H9195_RS17720 (3662664) 3662664..3663995 + 1332 WP_001183207.1 MFS transporter -
H9195_RS17725 (3664103) 3664103..3664633 + 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
H9195_RS17730 (3664626) 3664626..3666488 + 1863 WP_000377109.1 glutathione-regulated potassium-efflux system protein KefC -
H9195_RS17735 (3666680) 3666680..3667159 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
H9195_RS17740 (3667245) 3667245..3667478 + 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
H9195_RS17745 (3667481) 3667481..3667795 + 315 WP_001160966.1 CcdB family protein Toxin
H9195_RS17750 (3667792) 3667792..3668640 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
H9195_RS17755 (3668647) 3668647..3669024 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
H9195_RS17760 (3669027) 3669027..3669848 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
H9195_RS17765 (3669845) 3669845..3670834 - 990 WP_000241225.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
H9195_RS17770 (3670834) 3670834..3672120 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T171739 WP_001160966.1 NZ_CP060953:3667481-3667795 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T171739 NZ_CP078071:c965653-964757 [Burkholderia sp. EMB26]
ATGAGTGTCATCGACAAACGTGAACGCGACCGCACCGCGGCGTTCGCCGCGCTGCTCGGGCAGTTTCCCGAGCAGCGTTG
CGCGGCCGGCGCGGCGGGCACGATCGGTTATCGCGAGGCCGGCGCGCAGCATGCGGGCCGCGCGCTGCCCGTCGTGCTGC
TGCACGGGATCGGCTCGGGCGCCGCGTCGTGGGTCCGTCAGCTCGACACGCTGGGCGCGTCGCGGCGCGTGCTCGCGTGG
GATGCGCCCGGATACGGCGTGTCGACGCCCGTGCACGGCGCGTCGCCGGCCGCCGCCGATTACGCGGCAGCGCTGAACGC
GTGGCTCGAGGCACTCGGCATCGAACGCTGCGTCCTGGTCGGCCATTCGCTCGGCGCGATCATCGCGGGCGGCCTCGCCC
GCGTGACGCCCGCGCGGATCGCCGGCCTGCTGCTGGTGTCGCCCGCGGGCGGCTACGGCAGCGCACCGGCCGAGACCCGC
GAAGCGCGCCGCGACGCGCGGCTCGCGATGCTCGCGGAACTCGGCCCGGCCGGGCTCGCCGAGCAGCGCAGCGGCAACAT
GCTGTCCGGTTTCGCGAACGAGGAGGCGAAGGCATGGGTGCGCTGGAACATGGCGCGCATCGTGCCGGCCGGCTATGCGC
AGGCCACGCATCTGCTCGCGAACGCCGATCTCGCGGCCGATCTCGCCGGCTTCCGGGGCCGCACGGCCGTGGCCGTCGGC
GCGAACGACGCGATCACGCCGCCCGCCGCGTGCGAGCGGATCGCCGCGGCCGCGCACGTCGGGCTGCAGGTGATTCCGCA
GGCAGGGCACGCCGGCTATGTGGAAGCGCCGGCCGCGTATTCCGCGCTGATCGACTCGTTCTGCCGGCAGTGCGATACGC
AGTGGGGGCTGGCATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT171739 WP_000125568.1 NZ_CP060953:3667245-3667478 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT171739 NZ_CP078071:c964718-963861 [Burkholderia sp. EMB26]
ATGAGCGAACCGCTGCAACAAAACGAATCCGAAAACGCGAAGGCCGAAGCCAGCTACGTGGTGCCGGGCCTCGAACGCGG
GTTGCGGATTCTCGCCGAATTCTCGCCGCGCGAGCCCGTGCTCGGCGCGCCTGAACTGTCGAAGCGGCTCGGCATTCCGC
GCACGACGGTGTTCCGTCTGCTGCAGACGCTCGAATCGCTCGGCTTTCTCGAACGCGCGGACAAGGATCGCAACTACAAG
CTCGGCATCGCCGTGCTTCGGCTCGGCTTCGAATACCTGAGCTCGCTGGAGCTGACCGATCTCGGCCTGCCGGTGATCGA
AAGCCTGCGCGATGCGACCGGCTTCACGACGCACATCGTGATCCGCGACGGCCGCGACGTGGTGTTCGTCGCGAAGGCGC
AGAGCCAGTCGCCGGTGTTCAGTTCGATCCGCGTGAACGTCGGCACCCGGCTGCCCGCGCATGCAACGACGCACGGCCAC
GTGCTGATGGGCGACCTGTCGCTGAAGGAACTGCATGCGCTGTATCCGGAAGGCGTGCTGAACCGGATGACGAGCGCGAC
GCCGGAAACGGTCGACGCGCTGTACGAAGTGATCCGCGAGGATGCGCTGCGCGGCTACGGCGTCAGCAATTCGTCGTTCG
AGCGCGGGATCTCGGTCGTCACGGCGCCGGTGCGCAACGAGACGCAGAAGATCGTCGCTTGCATCACGGTGACGGTGCCG
CGTCCGGAGATCGACGCGGCGCTGATCGCGGACGGGCTGATCGACAAGGTGCAGCGCGCGGCGGCGGAACTGTCGCGGCG
GCTGAATTACCGCTCGGATGACGAGCACACGTTTCTCAAAGCTTTAGGACTCCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References