Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3243633..3244130 Replicon chromosome
Accession NZ_CP060953
Organism Escherichia coli strain EC8

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag H9195_RS15620 Protein ID WP_001173343.1
Coordinates 3243957..3244130 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag H9195_RS15615 Protein ID WP_001312331.1
Coordinates 3243633..3243929 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9195_RS15595 (3240362) 3240362..3241081 - 720 WP_001540474.1 two-component system response regulator DcuR -
H9195_RS15600 (3241078) 3241078..3242709 - 1632 WP_001216482.1 sensor histidine kinase -
H9195_RS15605 (3242890) 3242890..3243120 + 231 WP_000373293.1 4Fe-4S mono-cluster protein YjdI -
H9195_RS15610 (3243132) 3243132..3243404 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
H9195_RS15615 (3243633) 3243633..3243929 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
H9195_RS15620 (3243957) 3243957..3244130 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
H9195_RS15625 (3244249) 3244249..3245766 - 1518 WP_000003806.1 lysine--tRNA ligase -
H9195_RS15630 (3246003) 3246003..3247460 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3243633..3301293 57660


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T171730 WP_001173343.1 NZ_CP060953:3243957-3244130 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T171730 NZ_CP078069:c530995-530714 [Comamonas sp. NLF-1-9]
ATGCCCATTGTTTCCTTCAGGTGCCCAGACACTCAGCGCTTGTTCACCAGCGGCAAGACACGGCGTTTTTCCAGCTTCCA
GTCTGTGGCAGAGCGCAAGCTGGCACAACTGGATGCCGCCGTGACACTGGATTTTTTGCGCAGTCCGCCAGGCAATCGCC
TTGAGCTGCTGACGGGCGACCGCCAAGGGCAGCACAGCATCCGCATCAACGGCCAGTGGCGCGTGTGCTTTGTCTGGACG
GACAAAGGGCCCATGAATGTGGAAATCGTCGACTATCACTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT171730 WP_001312331.1 NZ_CP060953:3243633-3243929 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT171730 NZ_CP078069:c530688-530380 [Comamonas sp. NLF-1-9]
ATGGTCAAAAACGGCATGCGCCCCATCCACCCGGGCGAAATATTGCGTGAGGACTACCTCAAACCTCTGCAGCTCAGCGC
CAATGCGCTGGCCGGGCAACTGCACGTACCTGCATCGCGCATCAACGACATCGTGCTGGGGCGCCGGGGCATTACCGCCG
ATACGGCCTTGCGCCTGTGCCGCTTTTTTGGGGGTGACGCACGGTCATGGCTCAATCTGCAAACGGCTTACGATTTGCGC
GTGGCCGAGCTTGACGGGGCAGTGCAACAGGCCGTGCAGACGGTGCACCCCATGCTGCATCCGACCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References