Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1786454..1787108 Replicon chromosome
Accession NZ_CP060953
Organism Escherichia coli strain EC8

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag H9195_RS08680 Protein ID WP_000244781.1
Coordinates 1786454..1786861 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag H9195_RS08685 Protein ID WP_000354046.1
Coordinates 1786842..1787108 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9195_RS08660 (1782411) 1782411..1784144 - 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -
H9195_RS08665 (1784150) 1784150..1784860 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
H9195_RS08670 (1784885) 1784885..1785781 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
H9195_RS08675 (1785893) 1785893..1786414 + 522 WP_001055874.1 flavodoxin FldB -
H9195_RS08680 (1786454) 1786454..1786861 - 408 WP_000244781.1 protein YgfX Toxin
H9195_RS08685 (1786842) 1786842..1787108 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
H9195_RS08690 (1787351) 1787351..1788331 + 981 WP_000886078.1 tRNA-modifying protein YgfZ -
H9195_RS08695 (1788408) 1788408..1789067 - 660 WP_000250274.1 hemolysin III family protein -
H9195_RS08700 (1789231) 1789231..1789542 - 312 WP_001182959.1 N(4)-acetylcytidine aminohydrolase -
H9195_RS08705 (1789587) 1789587..1791020 + 1434 WP_001339296.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T171725 WP_000244781.1 NZ_CP060953:c1786861-1786454 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T171725 NZ_CP078069:255000-255290 [Comamonas sp. NLF-1-9]
ATGTTGGTCATCCACACCACTGAGACATTCGACCGCTGGTTCGAATCCCTGCGCGACAGGCGCGCGGCGCAACGTATACA
GGCGCGTATCGACCGGGCAGAAGACGGGAATTTCGGCGATTGCGAGCCCATTGGTGAGGGCGTCTCGGAAATGCGGATCC
ACCACGGCCCCGGCTATCGGGTTTATTTTGTGGGGCGCGGTATGGAGGTCGTGATCTTGCTGGCAGGCGGCAACAAAGCC
AGCCAAGGCAAGGACATCAAGACCGCCTTGGAACTGGCGCGACGACTATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT171725 WP_000354046.1 NZ_CP060953:c1787108-1786842 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT171725 NZ_CP078069:255306-255608 [Comamonas sp. NLF-1-9]
ATGGAACCCCTCAAACTGCGCAGGTGGGACAGCGCGGAGCATCTCAAGACCGAAGAGGACATGGTGCTGTATCTGCAAGC
CTGCATGGAAGAGGCGGGAGACGACGCCGCCTTCATCGCCAAGGCGCTTGGCAATGTCGCCCGCGCCCGTGGCATGACCC
AGCTCGCCAAGGAGACCGGTCTGGGCCGCGAAAGCCTCTACAAGGCGCTGTCGGGCGAAGGCAACCCGAGCTTTGGCACG
ATCCTCAAGGTCATGCACGCGCTGGGCATCAAGCTGCAGGCCCAGGCAGCGGACCCAGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References