Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1630526..1631109 Replicon chromosome
Accession NZ_CP060953
Organism Escherichia coli strain EC8

Toxin (Protein)


Gene name mazF Uniprot ID S1PVD8
Locus tag H9195_RS08055 Protein ID WP_000254749.1
Coordinates 1630526..1630861 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag H9195_RS08060 Protein ID WP_000581937.1
Coordinates 1630861..1631109 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9195_RS08040 (1626412) 1626412..1627710 - 1299 WP_000036723.1 phosphopyruvate hydratase -
H9195_RS08045 (1627798) 1627798..1629435 - 1638 WP_001774030.1 CTP synthase (glutamine hydrolyzing) -
H9195_RS08050 (1629663) 1629663..1630454 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
H9195_RS08055 (1630526) 1630526..1630861 - 336 WP_000254749.1 endoribonuclease MazF Toxin
H9195_RS08060 (1630861) 1630861..1631109 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
H9195_RS08065 (1631187) 1631187..1633421 - 2235 WP_000226797.1 GTP diphosphokinase -
H9195_RS08070 (1633469) 1633469..1634770 - 1302 WP_000046817.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12137.06 Da        Isoelectric Point: 8.7218

>T171724 WP_000254749.1 NZ_CP060953:c1630861-1630526 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKR
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T171724 NZ_CP078066:c6434013-6433852 [Bradyrhizobium arachidis]
ATGCGTAAGCCACCGAAGAATGTCGGTGCATCCGTTCGGGCCCGCCTGCTCGACCTTTCCAAGGAACGCAACGAGCCGTT
CGAGCTCATACTTACGCACTATGCCATCGAACGGCTGCTCTACCGGCTCAGCGTCAGCCAACATAAAACAGGTTTGTTTT
GA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT171724 WP_000581937.1 NZ_CP060953:c1631109-1630861 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT171724 NZ_CP078066:c6434653-6434006 [Bradyrhizobium arachidis]
ATGGAAAAGGAGCCGTCACGCCAGGAGGATCGCGCCACCTCGCTTCTTCGGAAGCAGGGGATGATGCGCCTGTCCGAGTT
CCACGAGCACGGCATCACGTCGGCGACCATTTCCCGAATGGAAGAAAGAGGTCTGCTCAATCAGCTCGCGCGTGGCCTTT
ATCAGCTGCCGGATGCTCCGCTGGATGGAAACCACTCTCTTGTCGTCGCCGCAAAGCTCATCCCCAACGGTGTTGTTTGC
TACGATTCCGCGCTTGCGTTTCACGAACTGACGGATCGCATTCCACCTTACGTTTGGATGGGTATCGGTCAACGCGATTG
GCGTCCTAAAATCTCTAGCCCCCGCATTGAGATCGTCCGCTTCAATCAGTCGGAATTTACCAGTGGCGTCGACCAGCACA
TTATTGAAAATGTGTCGGTTGCAATTTATAGGCCCGCCAAAACGATTGTGGACCTTTTCAAGAGCGGCAAGCGACAAAAA
GCTTTCTACGATTCAAATACCGGCTTCGCCCATGCTACACAGGCGATGAAAAAGGCTCTGCGCCTTCGTATGACTACGCC
CGCGGAAATTGCCCGGTTTGCCGTTCAGTCAAACGTCTGGGAAAAAATAGTTCAGCCGCGGCTGGAGGCGCTCACGATCA
ATGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PVD8

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References