Detailed information of TA system TA171709

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 1985..2544
• Replicon: plasmid pEC9-2
• Accession: NZ_CP060951
• Organism: Escherichia coli strain EC9

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YB49
• Locus tag: H9196_RS23605
• Protein ID: WP_000421259.1
• Coordinates: 2269..2544 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: H9196_RS23600
• Protein ID: WP_001178087.1
• Coordinates: 1985..2269 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9196_RS23595 (46)	46..1077	+	1032	WP_000907868.1	plasmid replication initiator RepA	-
H9196_RS23600 (1985)	1985..2269	+	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
H9196_RS23605 (2269)	2269..2544	+	276	WP_000421259.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H9196_RS23610 (2650)	2650..2853	+	204	Protein_3	hypothetical protein	-
H9196_RS23615 (3123)	3123..3389	-	267	WP_000627832.1	hypothetical protein	-
H9196_RS23620 (3389)	3389..3883	-	495	WP_015058884.1	hypothetical protein	-
H9196_RS23625 (3939)	3939..4541	-	603	WP_000521606.1	ProQ/FINO family protein	-
H9196_RS23630 (4717)	4717..5769	-	1053	WP_000655519.1	hypothetical protein	-
H9196_RS23635 (5982)	5982..6572	-	591	WP_001401589.1	hypothetical protein	-
H9196_RS23640 (6572)	6572..6826	-	255	WP_000343106.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..87111	87111

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10736.45 Da
• Isoelectric Point: 9.9324

>T171709 WP_000421259.1 NZ_CP060951:2269-2544 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T171709 NZ_CP078034:c27314-26898 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT171709 WP_001178087.1 NZ_CP060951:1985-2269 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT171709 NZ_CP078034:c27541-27311 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: U9YB49

Antitoxin

• AlphaFold DB: U9Y625